Microbial activities in deep subsurface environments

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Activities of microorganisms residing in terrestrial deep subsurface sediments were examined in forty-six sediment samples from three aseptically sampled boreholes. Radiolabeled time course experiments assessing in situ microbial activities were initiated within 30 minutes of core recovery. [{sup 14}C-1-] Acetate incorporation into lipids. [methyl-{sup 3}H-]thymidine incorporation into DNA, [{sup 14}C-2-]acetate and [{sup 14}C-U-]glucose mineralization in addition to microbial enrichment and enumeration studies were examined in surface and subsurface sediments. Surface soils contained the greatest biomass and activities followed by the shallow aquifer zones. Water saturated subsurface sediments exhibited three to four orders of magnitude greater activity and culturable microorganisms than ... continued below

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28 p.

Creation Information

Phelps, T. J.; Raione, E. G.; White, D. C. & Fliermans, C. B. Spring 1988.

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  • Savannah River Laboratory
    Publisher Info: E.I. DuPont de Nemours and Co., Savannah River Lab., Aiken, SC (United States)
    Place of Publication: Aiken, South Carolina

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Description

Activities of microorganisms residing in terrestrial deep subsurface sediments were examined in forty-six sediment samples from three aseptically sampled boreholes. Radiolabeled time course experiments assessing in situ microbial activities were initiated within 30 minutes of core recovery. [{sup 14}C-1-] Acetate incorporation into lipids. [methyl-{sup 3}H-]thymidine incorporation into DNA, [{sup 14}C-2-]acetate and [{sup 14}C-U-]glucose mineralization in addition to microbial enrichment and enumeration studies were examined in surface and subsurface sediments. Surface soils contained the greatest biomass and activities followed by the shallow aquifer zones. Water saturated subsurface sediments exhibited three to four orders of magnitude greater activity and culturable microorganisms than the dense clay zones. Regardless of depth, sediments which contained more than 20% clays exhibited the lowest activities and culturable microorganisms.

Physical Description

28 p.

Notes

OSTI as DE98007475

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  • Other Information: PBD: [1988]

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  • Other: DE98007475
  • Report No.: DP-MS--88-100
  • Grant Number: AC09-89SR18035;AC09-76SR00001
  • DOI: 10.2172/666219 | External Link
  • Office of Scientific & Technical Information Report Number: 666219
  • Archival Resource Key: ark:/67531/metadc708091

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • Spring 1988

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  • Sept. 12, 2015, 6:31 a.m.

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  • March 23, 2016, 12:14 p.m.

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Phelps, T. J.; Raione, E. G.; White, D. C. & Fliermans, C. B. Microbial activities in deep subsurface environments, report, Spring 1988; Aiken, South Carolina. (digital.library.unt.edu/ark:/67531/metadc708091/: accessed December 16, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.