Aequorin as a bioluminescent indicator for use in the determination of biomolecules in single cells. Final technical report

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Description

During this funding period, the laboratories of Drs. Anderson and Daunert have performed a considerable amount of work toward addressing the issues associated with small volume analysis necessary for single cell studies. In that respect, their research has been focused on (1) developing new assays that can be miniaturized and are suitable for small volume and single cell analysis; (2) fabricating pL-vials that simulate the volume of single cells and setting up instrumentation capable of low-volume detection; (3) developing reproducible and reliable microinjection techniques; (4) developing methods of analysis for biomolecules in the pL-vials and employing these assays in the ... continued below

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15 p.

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Daunert, Sylvia February 17, 2000.

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Description

During this funding period, the laboratories of Drs. Anderson and Daunert have performed a considerable amount of work toward addressing the issues associated with small volume analysis necessary for single cell studies. In that respect, their research has been focused on (1) developing new assays that can be miniaturized and are suitable for small volume and single cell analysis; (2) fabricating pL-vials that simulate the volume of single cells and setting up instrumentation capable of low-volume detection; (3) developing reproducible and reliable microinjection techniques; (4) developing methods of analysis for biomolecules in the pL-vials and employing these assays in the detection of biomolecules in single cells. The accomplishments attained in all these areas are described below. A total of 24 publications and 35 presentations have resulted from this work.

Physical Description

15 p.

Notes

OSTI as DE00755430

Medium: P; Size: 15 pages

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  • Other Information: PBD: 17 Feb 2000

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  • Report No.: none
  • Grant Number: FG02-95ER62010
  • DOI: 10.2172/755430 | External Link
  • Office of Scientific & Technical Information Report Number: 755430
  • Archival Resource Key: ark:/67531/metadc707513

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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Creation Date

  • February 17, 2000

Added to The UNT Digital Library

  • Sept. 12, 2015, 6:31 a.m.

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  • April 7, 2017, 2:56 p.m.

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Daunert, Sylvia. Aequorin as a bioluminescent indicator for use in the determination of biomolecules in single cells. Final technical report, report, February 17, 2000; United States. (digital.library.unt.edu/ark:/67531/metadc707513/: accessed November 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.