Cloning and sequencing of the trpE gene from Arthrobacter globiformis ATCC 8010 and several related subsurface Arthrobacter isolates

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Description

Tryptophan dependent mutants of Arthrobacter globiformis ATCC 8010 were isolated and trp genes were cloned by complementation and marker rescue of the auxotrophic strains. Rescue studies and preliminary sequence analysis reveal that at least the genes trpE, trpC, and trpB are clustered together in this organism. In addition, sequence analysis of the entire trpE gene, which encodes component I of anthranilate synthase, is described. Segments of the trpE gene from 17 subsurface isolates of Arthrobacter sp. were amplified by PCR and sequenced. The partial trpE sequences from the various strains were aligned and subjected to phylogenetic analysis. The data suggest ... continued below

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Medium: P; Size: 23 p.

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Chernova, T.; Viswanathan, V.K.; Austria, N. & Nichols, B.P. September 1, 1998.

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Description

Tryptophan dependent mutants of Arthrobacter globiformis ATCC 8010 were isolated and trp genes were cloned by complementation and marker rescue of the auxotrophic strains. Rescue studies and preliminary sequence analysis reveal that at least the genes trpE, trpC, and trpB are clustered together in this organism. In addition, sequence analysis of the entire trpE gene, which encodes component I of anthranilate synthase, is described. Segments of the trpE gene from 17 subsurface isolates of Arthrobacter sp. were amplified by PCR and sequenced. The partial trpE sequences from the various strains were aligned and subjected to phylogenetic analysis. The data suggest that in addition to single base changes, recombination and genetic exchange play a major role in the evolution of the Arthrobacter genome.

Physical Description

Medium: P; Size: 23 p.

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OSTI as DE98006375

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  • Other Information: PBD: [1998]

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  • Other: DE98006375
  • Report No.: DOE/ER/61679--T1
  • Grant Number: FG02-93ER61679
  • DOI: 10.2172/663530 | External Link
  • Office of Scientific & Technical Information Report Number: 663530
  • Archival Resource Key: ark:/67531/metadc707484

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  • September 1, 1998

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  • Sept. 12, 2015, 6:31 a.m.

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  • Nov. 4, 2015, 3:55 p.m.

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Chernova, T.; Viswanathan, V.K.; Austria, N. & Nichols, B.P. Cloning and sequencing of the trpE gene from Arthrobacter globiformis ATCC 8010 and several related subsurface Arthrobacter isolates, report, September 1, 1998; United States. (digital.library.unt.edu/ark:/67531/metadc707484/: accessed September 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.