Ethanol Production from Glucose and Xylose by Immobilized <i>Zymomonas mobilis</i> CP4(pZB5)

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Fermentation of glucose-xylose mixtures to ethanol was investigated in batch and continuous experiments using immobilized recombinant <i>Zymomonas mobilis</i> CP4(pZB5). This microorganism was immobilized by entrapment in k-carrageenan beads having a diameter of 1.5-2.5 mm. Batch experiments showed that the immobilized cells co-fermented glucose and xylose to ethanol and that the presence of glucose improved the xylose utilization rate. Batch fermentation of rice straw hydrolyzate containing 76 g/L glucose and 33.8 g/L xylose gave an ethanol concentration of 44.3 g/L after 24 hours, corresponding to a yeild of 0.46 g ethanol/g sugars. Comparable results were achieved with a synthetic sugar control. ... continued below

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Blanco, M.; Davison, B.H.; Krishnan, M.S.; Nghiem, n.P. & Shattuck, C.K. May 2, 1999.

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Fermentation of glucose-xylose mixtures to ethanol was investigated in batch and continuous experiments using immobilized recombinant <i>Zymomonas mobilis</i> CP4(pZB5). This microorganism was immobilized by entrapment in k-carrageenan beads having a diameter of 1.5-2.5 mm. Batch experiments showed that the immobilized cells co-fermented glucose and xylose to ethanol and that the presence of glucose improved the xylose utilization rate. Batch fermentation of rice straw hydrolyzate containing 76 g/L glucose and 33.8 g/L xylose gave an ethanol concentration of 44.3 g/L after 24 hours, corresponding to a yeild of 0.46 g ethanol/g sugars. Comparable results were achieved with a synthetic sugar control. Continuous fermentation runs were performed in a laboratory scale fluidized-bed bioreactor (FBR). Glucose-xylose feed mixtures were run through the FBR at residence times of 2 to 4 hours. Glucose conversion to ethanol was maintained above 98% in all continuous runs. Xylose conversion to ethanol was highest at 91.5% for a feed containing 50 g/L glucose-13 g/L xylose at a dilution rate of 0.24 h<sup>-1</sup>. The xylose conversion to ethanol decreased with increasing feed xylose concentration, dilution rate and age of the immobilized cells. Volumetric ethanol productivities in the range of 6.5 to 15.3 g/L-h were obtained.

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12 pages

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  • 21st Sympsoium on Biotechnology for Fuels and Chemicals, Ft. Collins, CO, May 2-6, 1999

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  • Other: DE00006930
  • Report No.: ORNL/CP-103176
  • Grant Number: AC05-96OR22464
  • Office of Scientific & Technical Information Report Number: 6930
  • Archival Resource Key: ark:/67531/metadc707417

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  • May 2, 1999

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  • Sept. 12, 2015, 6:31 a.m.

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  • Feb. 15, 2016, 12:20 p.m.

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Blanco, M.; Davison, B.H.; Krishnan, M.S.; Nghiem, n.P. & Shattuck, C.K. Ethanol Production from Glucose and Xylose by Immobilized <i>Zymomonas mobilis</i> CP4(pZB5), article, May 2, 1999; Oak Ridge, Tennessee. (digital.library.unt.edu/ark:/67531/metadc707417/: accessed October 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.