Rapid, potentially automatable, method extract biomarkers for HPLC/ESI/MS/MS to detect and identify BW agents

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Description

The program proposes to concentrate on the rapid recovery of signature biomarkers based on automated high-pressure, high-temperature solvent extraction (ASE) and/or supercritical fluid extraction (SFE) to produce lipids, nucleic acids and proteins sequentially concentrated and purified in minutes with yields especially from microeukaryotes, Gram-positive bacteria and spores. Lipids are extracted in higher proportions greater than classical one-phase, room temperature solvent extraction without major changes in lipid composition. High performance liquid chromatography (HPLC) with or without derivatization, electrospray ionization (ESI) and highly specific detection by mass spectrometry (MS) particularly with (MS){sup n} provides the detection, identification and because the signature lipid ... continued below

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8 p.

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White, D. C.; Burkhalter, R. S.; Smith, C. & Whitaker, K. W. December 1997.

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  • White, D. C. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology
  • Burkhalter, R. S.
  • Smith, C. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology
  • Whitaker, K. W. Microbial Insights, Inc., Rockford, TN (United States)

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Description

The program proposes to concentrate on the rapid recovery of signature biomarkers based on automated high-pressure, high-temperature solvent extraction (ASE) and/or supercritical fluid extraction (SFE) to produce lipids, nucleic acids and proteins sequentially concentrated and purified in minutes with yields especially from microeukaryotes, Gram-positive bacteria and spores. Lipids are extracted in higher proportions greater than classical one-phase, room temperature solvent extraction without major changes in lipid composition. High performance liquid chromatography (HPLC) with or without derivatization, electrospray ionization (ESI) and highly specific detection by mass spectrometry (MS) particularly with (MS){sup n} provides the detection, identification and because the signature lipid biomarkers are both phenotypic as well as genotypic biomarkers, insights into potential infectivity of BW agents. Feasibility has been demonstrated with detection, identification, and determination of infectious potential of Cryptosporidium parvum at the sensitivity of a single oocyst (which is unculturable in vitro) and accurate identification and prediction, pathogenicity, and drug-resistance of Mycobacteria spp.

Physical Description

8 p.

Notes

OSTI as DE98003517

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  • 1997 U.S. Army Edgewood Research, Development and Engineering Center scientific conference on chemical and biological defense research, Aberdeen Proving Ground, MD (United States), 18-21 Nov 1997

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  • Other: DE98003517
  • Report No.: ORNL/CP--97757
  • Report No.: CONF-971163--
  • Grant Number: AC05-96OR22464
  • Office of Scientific & Technical Information Report Number: 661538
  • Archival Resource Key: ark:/67531/metadc707287

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Creation Date

  • December 1997

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  • Sept. 12, 2015, 6:31 a.m.

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  • May 20, 2016, 1:50 p.m.

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White, D. C.; Burkhalter, R. S.; Smith, C. & Whitaker, K. W. Rapid, potentially automatable, method extract biomarkers for HPLC/ESI/MS/MS to detect and identify BW agents, article, December 1997; Tennessee. (digital.library.unt.edu/ark:/67531/metadc707287/: accessed December 9, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.