Opto-acoustic cell permeation

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Optically generated acoustic waves have been used to temporarily permeate biological cells. This technique may be useful for enhancing transfection of DNA into cells or enhancing the absorption of locally delivered drugs. A diode-pumped frequency-doubled Nd:YAG laser operating at kHz repetition rates was used to produce a series of acoustic pulses. An acoustic wave was formed via thermoelastic expansion by depositing laser radiation into an absorbing dye. Generated pressures were measured with a PVDF hydrophone. The acoustic waves were transmitted to cultured and plated cells. The cell media contained a selection of normally- impermeable fluorescent-labeled dextran dyes. Following treatment with ... continued below

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271 Kilobytes pages

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Visuri, S R & Heredia, N March 9, 2000.

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Optically generated acoustic waves have been used to temporarily permeate biological cells. This technique may be useful for enhancing transfection of DNA into cells or enhancing the absorption of locally delivered drugs. A diode-pumped frequency-doubled Nd:YAG laser operating at kHz repetition rates was used to produce a series of acoustic pulses. An acoustic wave was formed via thermoelastic expansion by depositing laser radiation into an absorbing dye. Generated pressures were measured with a PVDF hydrophone. The acoustic waves were transmitted to cultured and plated cells. The cell media contained a selection of normally- impermeable fluorescent-labeled dextran dyes. Following treatment with the opto-acoustic technique, cellular incorporation of dyes, up to 40,000 Molecular Weight, was noted. Control cells that did not receive opto-acoustic treatment had unremarkable dye incorporation. Uptake of dye was quantified via fluorescent microscopic analysis. Trypan Blue membrane exclusion assays and fluorescent labeling assays confirmed the vitality of cells following treatment. This method of enhanced drug delivery has the potential to dramatically reduce required drug dosages and associated side effects and enable revolutionary therapies.

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271 Kilobytes pages

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  • The International Society for Optical Engineering Photonics West, Photonics West'99 Symposium, SPIE, San Jose, CA (US), 01/23/2000--01/29/2000

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  • Report No.: UCRL-JC-137924
  • Report No.: KP1401030
  • Report No.: 98-LW-030
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 756840
  • Archival Resource Key: ark:/67531/metadc707145

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • March 9, 2000

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  • Sept. 12, 2015, 6:31 a.m.

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  • May 6, 2016, 2:50 p.m.

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Visuri, S R & Heredia, N. Opto-acoustic cell permeation, article, March 9, 2000; California. (digital.library.unt.edu/ark:/67531/metadc707145/: accessed November 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.