Analysis of hazardous biological material by MALDI mass spectrometry

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Description

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS) has become a valuable tool for analyzing microorganisms. The speed with which data can be obtained from MALDI-MS makes this a potentially important tool for biological health hazard monitoring and forensic applications. The excitement in the mass spectrometry community in this potential field of application is evident by the expanding list of research laboratories pursuing development of MALDI-MS for bacterial identification. Numerous research groups have demonstrated the ability to obtain unique MALDI-MS spectra from intact bacterial cells and bacterial cell extracts. The ability to differentiate strains of the same species has been investigated. ... continued below

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Medium: P; Size: 62 pages

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Wahl, KL; Jarman, KH; Valentine, NB; Kingsley, MT; Petersen, CE; Cebula, ST et al. March 21, 2000.

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Description

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS) has become a valuable tool for analyzing microorganisms. The speed with which data can be obtained from MALDI-MS makes this a potentially important tool for biological health hazard monitoring and forensic applications. The excitement in the mass spectrometry community in this potential field of application is evident by the expanding list of research laboratories pursuing development of MALDI-MS for bacterial identification. Numerous research groups have demonstrated the ability to obtain unique MALDI-MS spectra from intact bacterial cells and bacterial cell extracts. The ability to differentiate strains of the same species has been investigated. Reproducibility of MALDI-MS spectra from bacterial species under carefully controlled experimental conditions has also been demonstrated. Wang et al. have reported on interlaboratory reproducibility of the MALDI-MS analysis of several bacterial species. However, there are still issues that need to be addressed, including the careful control of experimental parameters for reproducible spectra and selection of optimal experimental parameters such as solvent and matrix.

Physical Description

Medium: P; Size: 62 pages

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OSTI as DE00752459

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  • Other Information: PBD: 21 Mar 2000

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  • Report No.: PNNL-13099
  • Report No.: 400480000
  • Grant Number: AC06-76RL01830
  • DOI: 10.2172/752459 | External Link
  • Office of Scientific & Technical Information Report Number: 752459
  • Archival Resource Key: ark:/67531/metadc707079

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  • March 21, 2000

Added to The UNT Digital Library

  • Sept. 12, 2015, 6:31 a.m.

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  • April 7, 2016, 1:08 p.m.

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Wahl, KL; Jarman, KH; Valentine, NB; Kingsley, MT; Petersen, CE; Cebula, ST et al. Analysis of hazardous biological material by MALDI mass spectrometry, report, March 21, 2000; Richland, Washington. (digital.library.unt.edu/ark:/67531/metadc707079/: accessed August 19, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.