Large scale solubilization of coal and bioconversion to utilizable energy. Technical progress report, January 1--March 31, 1996 Page: 3 of 18
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U
Introductirr
In orderta develop a system for a large scale: coal solubilization and its
bicconversiorr ta utilizable fuel, we plan to clone the genes encoding Neurospora
protein that facilitate depolymerization of coaL We also- plan to use desulfurizing
bacteria to remove the sulfur in situ and use other microorganisms to convert
biosolubilized coal into utilizable energy following an approach utilizing several
microorganisms (Faison, 1991). In addition the product of coal solubilized by fungus
will be characterized to determine their chemical nature- and the mechanism of reaction
catalyzed by fungal product during in vivo and in vitro solubilization by the fungus or
purified fungal protein.
Main Objectives
1. Cloning of Neurospora gene for coal depolymerization protein controlling
solubilization in different host cells, utilizing Neurospora plasmid and other
vector(s).
2. a. Development of a large scale electrophoretic separation of coal drived
products obtained after microbial solubilization.
b. Identification of the coal derived products obtained after biosolubilization
by Neurospora cultures or obtained after Neurospora enzyme catalyzed
reaction in in vitro by the wildtype and mutant enzymes.
3. Bioconversion of coal drived products into utilizable fuel.
4. Characterization of Neurospora wildtype and mutant CSA protein(s) involved in
solubilization of coal in order to assess the nature of the mechanism of
solubilization and the role of Neurospora proteins in this process.1
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Mishra, N. C. Large scale solubilization of coal and bioconversion to utilizable energy. Technical progress report, January 1--March 31, 1996, report, May 1, 1996; United States. (https://digital.library.unt.edu/ark:/67531/metadc706373/m1/3/: accessed April 25, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.