Application of spectral hole burning to the study of in vitro cellular systems Metadata

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Title

  • Main Title Application of spectral hole burning to the study of in vitro cellular systems

Creator

  • Author: Milanovich, Nebojsa
    Creator Type: Personal

Contributor

  • Sponsor: United States. Department of Energy.
    Contributor Type: Organization
    Contributor Info: US Department of Energy (United States)

Publisher

  • Name: Ames Laboratory
    Place of Publication: Iowa
    Additional Info: Ames Lab., IA (United States)

Date

  • Creation: 1999-11-08

Language

  • English

Description

  • Content Description: Chapter 1 of this thesis describes the various stages of tumor development and a multitude of diagnostic techniques used to detect cancer. Chapter 2 gives an overview of the aspects of hole burning spectroscopy important for its application to the study of cellular systems. Chapter 3 gives general descriptions of cellular organelles, structures, and physical properties that can serve as possible markers for the differentiation of normal and cancerous cells. Also described in Chapter 3 are the principles of cryobiology important for low temperature spectroscopy of cells, characterization of MCF-10F (normal) and MCF-7 (cancer) cells lines which will serve as model systems, and cellular characteristics of aluminum phthalocyanine tetrasulfonate (APT), which was used as the test probe. Chapters 4 and 5 are previously published papers by the author pertaining to the results obtained from the application of hole burning to the study of cellular systems. Chapter 4 presents the first results obtained by spectral hole burning of cellular systems and Chapter 5 gives results for the differentiation of MCF-10F and MCF-7 cells stained with APT by an external applied electric (Stark) field. A general conclusion is presented in Chapter 6. Appendices A and B provide additional characterization of the cell/probe model systems. Appendix A describes the uptake and subcellular distribution of APT in MCF-10F and MCF-7 cells and Appendix B compares the hole burning characteristics of APT in cells when the cells are in suspension and when they are examined while adhering to a glass coverslip. Appendix C presents preliminary results for a novel probe molecule, referred to as a molecular thumbtack, designed by the authors for use in future hole burning applications to cellular systems.
  • Physical Description: Medium: P; Size: 150 pages

Subject

  • Keyword: Tumor Cells
  • Keyword: Neoplasms
  • Keyword: Biological Markers
  • STI Subject Categories: 62 Radiology And Nuclear Medicine
  • Keyword: Molecular Structure
  • Keyword: Cell Differentiation
  • Keyword: Diagnostic Techniques
  • Keyword: Physical Properties
  • Keyword: Spectroscopy
  • Keyword: Cryobiology

Source

  • Other Information: TH: Thesis (Ph.D.); Submitted to Iowa State Univ., Ames, IA (US)

Collection

  • Name: Office of Scientific & Technical Information Technical Reports
    Code: OSTI

Institution

  • Name: UNT Libraries Government Documents Department
    Code: UNTGD

Resource Type

  • Thesis or Dissertation

Format

  • Text

Identifier

  • Report No.: IS-T-1873
  • Grant Number: W-7405-ENG-82
  • Office of Scientific & Technical Information Report Number: 754784
  • Archival Resource Key: ark:/67531/metadc704990

Note

  • Display Note: OSTI as DE00754784
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