HIV transcription is induced in dying cells

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Description

Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. 14 refs., 4 figs., 1 tab.

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19 p.

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Woloschak, G.E.; Chang-Liu, Chin-Mei & Schreck, S. June 1, 1995.

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Description

Using HeLa cells stably transfected with an HIV-LTR-CAT construct, we demonstrated a peak in CAT induction that occurs in viable (but not necessarily cell-division-competent) cells 24 h following exposure to some cell-killing agents. {gamma} rays were the only cell-killing agent which did not induce HIV transcription; this can be attributed to the fact that {gamma}-ray-induced apoptotic death requires functional p53, which is not present in HeLa cells. For all other agents, HIV-LTR induction was dose-dependent and correlated with the amount of cell killing that occurred in the culture. 14 refs., 4 figs., 1 tab.

Physical Description

19 p.

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OSTI as DE95012211

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  • Other Information: PBD: [1995]

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  • Other: DE95012211
  • Report No.: ANL/CMB/PP--85889
  • Grant Number: W-31109-ENG-38
  • DOI: 10.2172/74117 | External Link
  • Office of Scientific & Technical Information Report Number: 74117
  • Archival Resource Key: ark:/67531/metadc704763

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  • June 1, 1995

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  • Sept. 12, 2015, 6:31 a.m.

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  • Dec. 16, 2015, 12:37 p.m.

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Woloschak, G.E.; Chang-Liu, Chin-Mei & Schreck, S. HIV transcription is induced in dying cells, report, June 1, 1995; Illinois. (digital.library.unt.edu/ark:/67531/metadc704763/: accessed September 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.