Applications of Strand-Specific in situ Hybridization

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Description

This is the final report of a three-year, Laboratory-Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). Fluorescence in situ hybridization (FISH) is used to determine the location of specific DNA sequences on chromosomes. It is an effective tool in genomic mapping and is finding increasing use in medical diagnosis. A ''strand-specific'' version of FISH has been developed in the Life Sciences Division of LANL. The new procedure, named CO-FISH, reveals not only location but also the 5'-to-3'direction of a target sequence, such as the sense strand of a gene. This project was designed to investigate ... continued below

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7 p.

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Goodwin, E.H.; Meyne, J.; Bailey, S.M.; Quigley, D.; Smith, L. & Tennyson, R. January 1, 1997.

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Description

This is the final report of a three-year, Laboratory-Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). Fluorescence in situ hybridization (FISH) is used to determine the location of specific DNA sequences on chromosomes. It is an effective tool in genomic mapping and is finding increasing use in medical diagnosis. A ''strand-specific'' version of FISH has been developed in the Life Sciences Division of LANL. The new procedure, named CO-FISH, reveals not only location but also the 5'-to-3'direction of a target sequence, such as the sense strand of a gene. This project was designed to investigate applications of the new technique. Strand-specific FISH was found to be useful and informative for genomic mapping of repetitive DNA sequences. The method provide a valuable new tool for investigating the mechanisms of aneuploidy inducing agents and the cytogenetic phenomena called lateral asymmetry. Finally, using strand-specific FISH, the authors were able to detect certain types of chromosome aberrations (isochromosomes, inversions and Robertsonian translocations) that can be difficult to observe with standard techniques.

Physical Description

7 p.

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OSTI as DE00758749

Medium: P; Size: 7 pages

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  • Other Information: PBD: 1 Jan 1997

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  • Report No.: LA-UR-97-2246
  • Grant Number: W-7405-ENG-36
  • DOI: 10.2172/758749 | External Link
  • Office of Scientific & Technical Information Report Number: 758749
  • Archival Resource Key: ark:/67531/metadc704014

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Office of Scientific & Technical Information Technical Reports

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  • January 1, 1997

Added to The UNT Digital Library

  • Sept. 12, 2015, 6:31 a.m.

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  • April 11, 2017, 3:08 p.m.

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Goodwin, E.H.; Meyne, J.; Bailey, S.M.; Quigley, D.; Smith, L. & Tennyson, R. Applications of Strand-Specific in situ Hybridization, report, January 1, 1997; New Mexico. (digital.library.unt.edu/ark:/67531/metadc704014/: accessed June 18, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.