DNA and chromosome breaks induced by {sup 123}I-estrogen in CHO cells

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The effects of the Auger electron-emitting isotope I-123, covalently bound to estrogen, on DNA single- and double-strand breakage and on chromosome breakage was determined in estrogen positive Chinese hamster ovary (CHO-ER) cells. Exposure to the {sup 123}I-estrogen induced both single- and double-strand breaks with a ratio of single- to double-strand breaks of 2.2. The corresponding ratio with {sup 60}Co gamma rays was 15.6. The dose-response was biphasic suggesting that either receptor sites are saturated at high does, or that there is a nonrandom distribution of breaks induced by the {sup 123}I-estrogen. The {sup 123}I-estrogen treatment induced chromosome aberrations with an ... continued below

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31 p.

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Schwartz, J.L.; Mustafi, R.; Hughes, A. & DeSombre, E.R. July 1, 1997.

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The effects of the Auger electron-emitting isotope I-123, covalently bound to estrogen, on DNA single- and double-strand breakage and on chromosome breakage was determined in estrogen positive Chinese hamster ovary (CHO-ER) cells. Exposure to the {sup 123}I-estrogen induced both single- and double-strand breaks with a ratio of single- to double-strand breaks of 2.2. The corresponding ratio with {sup 60}Co gamma rays was 15.6. The dose-response was biphasic suggesting that either receptor sites are saturated at high does, or that there is a nonrandom distribution of breaks induced by the {sup 123}I-estrogen. The {sup 123}I-estrogen treatment induced chromosome aberrations with an efficiency of about 1 aberration for each 1,000 disintegrations per cell. This corresponds to the mean lethal dose of {sup 123}I-estrogen for these cells suggesting that the lethal event induced by the Auger electron emitter bound to estrogen is a chromosome aberration. Most of the chromosome-type aberrations were dicentrics and rings, suggesting that {sup 123}I-estrogen-induced chromosome breaks are rejoined. The F-ratio, the ratio of dicentrics to centric rings, was 5.8 {plus_minus} 1.7, which is similar to that seen with high LET radiations. Their results suggest that I-123 bound to estrogen is an efficient clastogenic agent, that the cytotoxic damage produced by I-123 bound to estrogen is very like high LET-induced damage, and the I-123 in the estrogen-receptor-DNA complex is probably in close proximity to the sugar-phosphate backbone of the DNA.

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31 p.

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INIS; OSTI as DE97007855

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  • Other Information: PBD: [1997]

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  • Other: DE97007855
  • Report No.: ANL/CMB/PP--87839
  • Grant Number: W-31109-ENG-38
  • DOI: 10.2172/505324 | External Link
  • Office of Scientific & Technical Information Report Number: 505324
  • Archival Resource Key: ark:/67531/metadc697597

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  • July 1, 1997

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  • Aug. 14, 2015, 8:43 a.m.

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  • Dec. 16, 2015, 6:45 p.m.

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Schwartz, J.L.; Mustafi, R.; Hughes, A. & DeSombre, E.R. DNA and chromosome breaks induced by {sup 123}I-estrogen in CHO cells, report, July 1, 1997; Illinois. (digital.library.unt.edu/ark:/67531/metadc697597/: accessed September 20, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.