Biophysical and biological factors determining the ability to achieve long-term cryobiological preservation Metadata

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Title

  • Main Title Biophysical and biological factors determining the ability to achieve long-term cryobiological preservation

Creator

  • Author: Mazur, P.
    Creator Type: Personal
    Creator Info: Oak Ridge National Lab., TN (United States). Life Sciences Div.

Contributor

  • Sponsor: United States. Department of Energy.
    Contributor Type: Organization
    Contributor Info: USDOE, Washington, DC (United States)
  • Sponsor: National Insts. of Health, Bethesda, MD (United States)
    Contributor Type: Organization
  • Sponsor: National Science Foundation (U.S.)
    Contributor Type: Organization
    Contributor Info: National Science Foundation, Washington, DC (United States)

Publisher

  • Name: Oak Ridge National Laboratory
    Place of Publication: Tennessee
    Additional Info: Oak Ridge National Lab., TN (United States)

Date

  • Creation: 1997-12-01

Language

  • English

Description

  • Content Description: The BESTCapsule will maintain appropriate biological specimens for decades or centuries at cryogenic temperatures in the living state. Maintenance at temperatures below {approximately} {minus}140 C is not a problem. No ordinary chemical reactions in aqueous solutions can occur. The only source of damage will be the slow accumulation of physical damage to DNA from background ionizing radiation. But this source of damage should not become serious in less than a millennium. Rather, the main problem in cryopreservation is to devise procedures for cooling the biological specimens to {minus}196 C and returning them to normal temperatures without inflicting lethal injury. Regardless of the cell type, there are certain encompassing biophysical factors and constraints that determine whether they will survive or die during freezing and thawing. Superimposed on these may be special biological factors that apply to specific cell types. This paper will emphasize the former and give illustrative examples of the latter.
  • Physical Description: 8 p.

Subject

  • Keyword: Storage
  • Keyword: Biological Materials
  • Keyword: Plant Cells
  • Keyword: Freezing
  • Keyword: Preservation
  • Keyword: Thawing
  • Keyword: Animal Cells
  • Keyword: Animal Tissues
  • Keyword: Cryobiology
  • Keyword: Plant Tissues
  • STI Subject Categories: 55 Biology And Medicine, Basic Studies
  • Keyword: Cryogenics

Source

  • Conference: BESTCapsule 2001 workshop, Osaka (Japan), 2-6 Nov 1997

Collection

  • Name: Office of Scientific & Technical Information Technical Reports
    Code: OSTI

Institution

  • Name: UNT Libraries Government Documents Department
    Code: UNTGD

Resource Type

  • Article

Format

  • Text

Identifier

  • Other: DE98001256
  • Report No.: ORNL/CP--95102
  • Report No.: CONF-971178--
  • Grant Number: AC05-96OR22464
  • Office of Scientific & Technical Information Report Number: 554808
  • Archival Resource Key: ark:/67531/metadc696846

Note

  • Display Note: OSTI as DE98001256
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