Human macrophage differentiation involves an interaction between integrins and fibronectin

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The authors have examined the role of integrins and extracellular matrix (ECM) proteins in macrophage differentiation of (1) human HL-60 myeloid leukemia cells induced by phorbol 12-myristate 13-acetate (PMA) and (2) human peripheral blood monocytes induced by either PMA or macrophage-colony stimulating factor (M-CSF). Increased {beta}{sub 1} integrin and fibronectin (FN) gene expression was observed in PMA-treated HL-60 cells and PMA- or M-CSF-treated monocytes, even at a time preceding the manifestation of macrophage markers. Treated HL-60 cells and monocytes also released and deposited FN on the culture dishes. An HL-60 cell variant, HL-525, which is deficient in protein kinase C ... continued below

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35 p.

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Laouar, A.; Chubb, C.B.H.; Collart, F. & Huberman, E. March 14, 1997.

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Description

The authors have examined the role of integrins and extracellular matrix (ECM) proteins in macrophage differentiation of (1) human HL-60 myeloid leukemia cells induced by phorbol 12-myristate 13-acetate (PMA) and (2) human peripheral blood monocytes induced by either PMA or macrophage-colony stimulating factor (M-CSF). Increased {beta}{sub 1} integrin and fibronectin (FN) gene expression was observed in PMA-treated HL-60 cells and PMA- or M-CSF-treated monocytes, even at a time preceding the manifestation of macrophage markers. Treated HL-60 cells and monocytes also released and deposited FN on the culture dishes. An HL-60 cell variant, HL-525, which is deficient in protein kinase C {beta} (PKC{beta}) and resistant to PMA-induced differentiation, failed to express FN after PMA treatment. Restoration of PKC{beta} resulted in PMA-induced FN gene expression and macrophage differentiation. The macrophage phenotype induced in HL-60 cells or monocytes was attenuated by anti-{beta}{sub 1} integrin or anti-FN MAbs. The authors suggest that macrophage differentiation involves activation of PKC and expression of specific integrins and ECM proteins. The stimulated cells, through their integrins, attach and spread on these substrates by binding to the deposited ECM proteins. This attachment and spreading in turn, through integrin signaling, leads to the macrophage phenotype.

Physical Description

35 p.

Notes

OSTI as DE97008258

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  • Other Information: PBD: 14 Mar 1997

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  • Other: DE97008258
  • Report No.: ANL/CMB/PP--92848
  • Grant Number: W-31109-ENG-38
  • DOI: 10.2172/515532 | External Link
  • Office of Scientific & Technical Information Report Number: 515532
  • Archival Resource Key: ark:/67531/metadc696604

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • March 14, 1997

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  • Aug. 14, 2015, 8:43 a.m.

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  • Dec. 16, 2015, 12:37 p.m.

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Laouar, A.; Chubb, C.B.H.; Collart, F. & Huberman, E. Human macrophage differentiation involves an interaction between integrins and fibronectin, report, March 14, 1997; Illinois. (digital.library.unt.edu/ark:/67531/metadc696604/: accessed October 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.