Relationship between the adhesive properties of bacteria and their transport and colonization in the subsurface environment. Final technical report

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Description

The adhesion of bacteria to sediment particle or rock surfaces considerably effects their transport in subsurface environments. This research focuses on the macromolecular properties of bacteria that determine their adhesiveness and on the significance of adhesion in transport of subsurface bacteria. Specific objectives include (1) to obtain adhesion mutants of subsurface Pseudomonas species altered in surface adhesives; (2) to determine alterations in adhesives in selected mutants; (3) to evaluate the effect of adhesiveness on transport and long-term distribution and colonization of bacteria in porous media. Primary methods will be tranposon mutagenesis to generate adhesion mutants, biochemical analyses of cell surface ... continued below

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5 p.

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Fletcher, M. March 13, 1997.

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Description

The adhesion of bacteria to sediment particle or rock surfaces considerably effects their transport in subsurface environments. This research focuses on the macromolecular properties of bacteria that determine their adhesiveness and on the significance of adhesion in transport of subsurface bacteria. Specific objectives include (1) to obtain adhesion mutants of subsurface Pseudomonas species altered in surface adhesives; (2) to determine alterations in adhesives in selected mutants; (3) to evaluate the effect of adhesiveness on transport and long-term distribution and colonization of bacteria in porous media. Primary methods will be tranposon mutagenesis to generate adhesion mutants, biochemical analyses of cell surface polymers, and the use of laboratory columns containing subsurface materials to study the distribution and transport of bacteria along flow paths over time.

Physical Description

5 p.

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OSTI as DE97008012

Medium: P; Size: 5 p.

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  • Other Information: PBD: 13 Mar 1997

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  • Other: DE97008012
  • Report No.: DOE/ER/61814--T1
  • Grant Number: FG02-94ER61814
  • DOI: 10.2172/515528 | External Link
  • Office of Scientific & Technical Information Report Number: 515528
  • Archival Resource Key: ark:/67531/metadc696578

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  • March 13, 1997

Added to The UNT Digital Library

  • Aug. 14, 2015, 8:43 a.m.

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  • April 13, 2017, 12:28 p.m.

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Fletcher, M. Relationship between the adhesive properties of bacteria and their transport and colonization in the subsurface environment. Final technical report, report, March 13, 1997; United States. (digital.library.unt.edu/ark:/67531/metadc696578/: accessed August 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.