Procedures for two-dimensional electrophoresis of proteins

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High-resolution two-dimensional gel electrophoresis (2DE) of proteins, using isoelectric focusing in the first dimension and sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) in the second, was first described in 1975. In the 20 years since those publications, numerous modifications of the original method have evolved. The ISO-DALT system of 2DE is a high-throughput approach that has stood the test of time. The problem of casting many isoelectric focusing gels and SDS-PAGE slab gels (up to 20) in a reproducible manner has been solved by the use of the techniques and equipment described in this manual. The ISO-DALT system of two-dimensional gel ... continued below

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90 p.

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Tollaksen, S.L. & Giometti, C.S. October 1, 1996.

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Description

High-resolution two-dimensional gel electrophoresis (2DE) of proteins, using isoelectric focusing in the first dimension and sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) in the second, was first described in 1975. In the 20 years since those publications, numerous modifications of the original method have evolved. The ISO-DALT system of 2DE is a high-throughput approach that has stood the test of time. The problem of casting many isoelectric focusing gels and SDS-PAGE slab gels (up to 20) in a reproducible manner has been solved by the use of the techniques and equipment described in this manual. The ISO-DALT system of two-dimensional gel electrophoresis originated in the late 1970s and has been modified many times to improve its high-resolution, high-throughput capabilities. This report provides the detailed procedures used with the current ISO-DALT system to prepare, run, stain, and photograph two-dimensional gels for protein analysis.

Physical Description

90 p.

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OSTI as DE97003102

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  • Other Information: PBD: Oct 1996

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  • Other: DE97003102
  • Report No.: ANL--96/19
  • Grant Number: W-31109-ENG-38
  • DOI: 10.2172/505307 | External Link
  • Office of Scientific & Technical Information Report Number: 505307
  • Archival Resource Key: ark:/67531/metadc695489

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  • October 1, 1996

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  • Aug. 14, 2015, 8:43 a.m.

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  • Dec. 15, 2015, 12:01 p.m.

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Tollaksen, S.L. & Giometti, C.S. Procedures for two-dimensional electrophoresis of proteins, report, October 1, 1996; Illinois. (digital.library.unt.edu/ark:/67531/metadc695489/: accessed August 16, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.