Production of ethanol from starch by co-immobilized Zymomonas mobilis -- Glucoamylase in a fluidized-bed reactor

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The production of ethanol from starch was studied in a fluidized-bed reactor (FBR) using co-immobilized Zymomonas mobilis and glucoamylase. The FBR was a glass column of 2.54 cm in diameter and 120 cm in length. The Z. mobilis and glucoamylase were co-immobilized within small uniform beads (1.2 to 2.5 mm diameter) of {kappa}-carrageenan. The substrate for ethanol production was a soluble starch. Light steep water was used as the complex nutrient source. The experiments were performed at 35 C and pH range 4.0 to 5.5. The substrate concentrations ranged from 40 to 185 g/L and the feed rates from 10 ... continued below

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18 p.

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Sun, M.Y.; Davison, B.H.; Bienkowski, P.R.; Nghiem, N.P. & Webb, O. August 1, 1997.

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The production of ethanol from starch was studied in a fluidized-bed reactor (FBR) using co-immobilized Zymomonas mobilis and glucoamylase. The FBR was a glass column of 2.54 cm in diameter and 120 cm in length. The Z. mobilis and glucoamylase were co-immobilized within small uniform beads (1.2 to 2.5 mm diameter) of {kappa}-carrageenan. The substrate for ethanol production was a soluble starch. Light steep water was used as the complex nutrient source. The experiments were performed at 35 C and pH range 4.0 to 5.5. The substrate concentrations ranged from 40 to 185 g/L and the feed rates from 10 to 37 mL/min. Under relaxed sterility conditions, the FBR was successfully operated for a period of 22 days, during which no contamination or structural failure of the biocatalyst beads was observed. Maximum volumetric productivity of 38 g ethanol/L-h, which was 76% of the theoretical value, was obtained. Typical ethanol volumetric productivity was in the range of 15 to 20 g/L-h. The average yield was 0.51 g ethanol/g substrate consumed, which was 90% of the theoretical yield. Very low levels of glucose were observed in the reactor, indicating that starch hydrolysis was the rate-limiting step.

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18 p.

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OSTI as DE97007704

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  • 19. symposium on biotechnology for fuels and chemicals, Colorado Springs, CO (United States), 4-8 May 1997

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  • Other: DE97007704
  • Report No.: CONF-9705112--3
  • Grant Number: AC05-96OR22464
  • Office of Scientific & Technical Information Report Number: 510345
  • Archival Resource Key: ark:/67531/metadc694309

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  • August 1, 1997

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  • Aug. 14, 2015, 8:43 a.m.

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  • Jan. 22, 2016, 2 p.m.

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Sun, M.Y.; Davison, B.H.; Bienkowski, P.R.; Nghiem, N.P. & Webb, O. Production of ethanol from starch by co-immobilized Zymomonas mobilis -- Glucoamylase in a fluidized-bed reactor, article, August 1, 1997; Tennessee. (digital.library.unt.edu/ark:/67531/metadc694309/: accessed September 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.