Pathway engineering to improve ethanol production by thermophilic bacteria Metadata

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Title

  • Main Title Pathway engineering to improve ethanol production by thermophilic bacteria

Creator

  • Author: Lynd, L. R.
    Creator Type: Personal

Contributor

  • Sponsor: United States. Department of Energy. Office of Energy Efficiency and Renewable Energy.
    Contributor Type: Organization
    Contributor Info: USDOE Assistant Secretary for Energy Efficiency and Renewable Energy, Washington, DC (United States)
  • Sponsor: National Science Foundation (U.S.)
    Contributor Type: Organization
    Contributor Info: National Science Foundation, Washington, DC (United States)

Publisher

  • Name: Dartmouth Coll., Hanover, NH (United States). Thayer School of Engineering
    Place of Publication: United States

Date

  • Creation: 1998-12

Language

  • English

Description

  • Content Description: Continuation of a research project jointly funded by the NSF and DOE is proposed. The primary project goal is to develop and characterize strains of C. thermocellum and C. thermosaccharolyticum having ethanol selectivity similar to more convenient ethanol-producing organisms. An additional goal is to document the maximum concentration of ethanol that can be produced by thermophiles. These goals build on results from the previous project, including development of most of the genetic tools required for pathway engineering in the target organisms. As well, we demonstrated that the tolerance of C. thermosaccharolyticum to added ethanol is sufficiently high to allow practical utilization should similar tolerance to produced ethanol be demonstrated, and that inhibition by neutralizing agents may explain the limited concentrations of ethanol produced in studies to date. Task 1 involves optimization of electrotransformation, using either modified conditions or alternative plasmids to improve upon the low but reproducible transformation, frequencies we have obtained thus far.
  • Physical Description: 35 p.

Subject

  • Keyword: Thermophilic Conditions
  • Keyword: Biomass
  • Keyword: Conversion
  • Keyword: Clostridium Thermocellum
  • STI Subject Categories: 09 Biomass Fuels
  • Keyword: Bioconversion
  • Keyword: Plasmids
  • STI Subject Categories: 55 Biology And Medicine, Basic Studies
  • Keyword: Clostridium Thermosaccharolyticum
  • Keyword: Ethanol
  • Keyword: Bacteria
  • Keyword: Tolerance

Source

  • Other Information: PBD: [1998]

Collection

  • Name: Office of Scientific & Technical Information Technical Reports
    Code: OSTI

Institution

  • Name: UNT Libraries Government Documents Department
    Code: UNTGD

Resource Type

  • Report

Format

  • Text

Identifier

  • Other: DE98000723
  • Report No.: DOE/CE/50364--T1
  • Grant Number: AI01-93CE50364
  • DOI: 10.2172/576095
  • Office of Scientific & Technical Information Report Number: 576095
  • Archival Resource Key: ark:/67531/metadc693906

Note

  • Display Note: OSTI as DE98000723
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