Pathway engineering to improve ethanol production by thermophilic bacteria

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Continuation of a research project jointly funded by the NSF and DOE is proposed. The primary project goal is to develop and characterize strains of C. thermocellum and C. thermosaccharolyticum having ethanol selectivity similar to more convenient ethanol-producing organisms. An additional goal is to document the maximum concentration of ethanol that can be produced by thermophiles. These goals build on results from the previous project, including development of most of the genetic tools required for pathway engineering in the target organisms. As well, we demonstrated that the tolerance of C. thermosaccharolyticum to added ethanol is sufficiently high to allow practical ... continued below

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35 p.

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Lynd, L. R. December 1998.

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Description

Continuation of a research project jointly funded by the NSF and DOE is proposed. The primary project goal is to develop and characterize strains of C. thermocellum and C. thermosaccharolyticum having ethanol selectivity similar to more convenient ethanol-producing organisms. An additional goal is to document the maximum concentration of ethanol that can be produced by thermophiles. These goals build on results from the previous project, including development of most of the genetic tools required for pathway engineering in the target organisms. As well, we demonstrated that the tolerance of C. thermosaccharolyticum to added ethanol is sufficiently high to allow practical utilization should similar tolerance to produced ethanol be demonstrated, and that inhibition by neutralizing agents may explain the limited concentrations of ethanol produced in studies to date. Task 1 involves optimization of electrotransformation, using either modified conditions or alternative plasmids to improve upon the low but reproducible transformation, frequencies we have obtained thus far.

Physical Description

35 p.

Notes

OSTI as DE98000723

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  • Other Information: PBD: [1998]

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  • Other: DE98000723
  • Report No.: DOE/CE/50364--T1
  • Grant Number: AI01-93CE50364
  • DOI: 10.2172/576095 | External Link
  • Office of Scientific & Technical Information Report Number: 576095
  • Archival Resource Key: ark:/67531/metadc693906

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Office of Scientific & Technical Information Technical Reports

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  • December 1998

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  • Aug. 14, 2015, 8:43 a.m.

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  • Nov. 13, 2015, 9:50 p.m.

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Lynd, L. R. Pathway engineering to improve ethanol production by thermophilic bacteria, report, December 1998; United States. (digital.library.unt.edu/ark:/67531/metadc693906/: accessed April 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.