Electrospray/Ion Trap Mass Spectrometry for the Detection and Identification of Organisms

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Description

Current electrospray ion trap methodology for rapid mixture analysis of proteins used for the identification of microorganisms is described. Development of ion/ion reaction techniques (e.g. reactions of multiply-charged protein cations with singly-charged anions) from both a fundamental and practical approach are presented, detailing the necessary steps and considerations involved in complex mixture analysis. Data describing the reduction of the initial charge states of electrospray ions to arbitrarily low values, the utility of ion/ion reactions for mixture separation on the millisecond time scale, and effects of excess singly-charged reactants on detection and storage efficiency are illustrated.

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8 p.

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McLuckey, Scott A. & Stephenson, James L., Jr. December 31, 1997.

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Description

Current electrospray ion trap methodology for rapid mixture analysis of proteins used for the identification of microorganisms is described. Development of ion/ion reaction techniques (e.g. reactions of multiply-charged protein cations with singly-charged anions) from both a fundamental and practical approach are presented, detailing the necessary steps and considerations involved in complex mixture analysis. Data describing the reduction of the initial charge states of electrospray ions to arbitrarily low values, the utility of ion/ion reactions for mixture separation on the millisecond time scale, and effects of excess singly-charged reactants on detection and storage efficiency are illustrated.

Physical Description

8 p.

Notes

OSTI as DE98000317

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  • 1. joint services workshop on biological mass spectrometry, Baltimore, MD (United States), 28-30 Jul 1997

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  • Other: DE98000317
  • Report No.: ORNL/CP--94409
  • Report No.: CONF-9707102--
  • Grant Number: AC05-96OR22464
  • Office of Scientific & Technical Information Report Number: 622805
  • Archival Resource Key: ark:/67531/metadc691985

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  • December 31, 1997

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  • Aug. 14, 2015, 8:43 a.m.

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  • Jan. 21, 2016, 8:10 p.m.

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McLuckey, Scott A. & Stephenson, James L., Jr. Electrospray/Ion Trap Mass Spectrometry for the Detection and Identification of Organisms, article, December 31, 1997; Tennessee. (digital.library.unt.edu/ark:/67531/metadc691985/: accessed October 15, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.