Comparative evolution of the recA gene of surface and deep subsurface microorganisms (an evolutionary clock of intermediate rate). Final report Page: 1 of 8
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405 744 7487 P.02
OSU GRANTS & CONTRACTS
RESEARCH INFORMATION MANAGEMENT SYSTEM
Award Information - ER61680
ID: --- ER61680/0000173
Robert V. Miller 405-744-7180
Institution: Oklahoma State University
Title: Comparative Evolution of the Reca Gene of
Surface and Deep Subsurface Microorganisms
(An Evolutionary Clock of Intermediate Rare)
ER Division: ER-74
Program Manager: Frank J. Wobber (301)903-5549
Research Areas: Cleanup
Most recent report of results to date:
Demonstration of the Effects of Mutation in the recA gene of
Pseudomonas aeruginosa. Identification of the Nucleotide Base
Changes in Two recA Alleles with Mutant Phenotypes.
Because of the ability of the RecA protein product to maintain both
DNA integrity and increase genetic diversity, this gene may be
essential to the survival of microorganisms following the damaging
effects of numerous environmental stresses such as exposure to solar
UV radiation, exposure to gamma radiation, starvation, and changing
environments. While the various activities and amino-acid sequence of
RecA have been highly conserved among the eubacteria and archaea,
little is known as to whether a strict structure-function relationship has
been conserved. In other words, are the same regions of this highly
plastic, functionally heterogeneous protein involved in the same
catalytic capacities throughout the bacterial kingdom? While it is
reasonable to assume that this type of conservation has also occurred,
we felt it necessary to test the assumption by demonstrating that
mutations in different genera of bacteria which eliminate similar
functions (i.e., lead to similar phenotypes) are caused by changes in
the amino-acid sequence in the same regions of their RecA proteins.
Therefore, we located tie changes in nucleotide sequence in two recA
mutants of P. aemuginosa which displayed mutant phenotypes in
recombination and UV resistance. Our assumption was that if
structure-function relationships held, these mutations would be found
in areas already identified as essential for the function of the E. coli
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Miller, R.V. Comparative evolution of the recA gene of surface and deep subsurface microorganisms (an evolutionary clock of intermediate rate). Final report, report, April 1, 1998; United States. (digital.library.unt.edu/ark:/67531/metadc689865/m1/1/: accessed January 17, 2019), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.