Development of BAC libraries and integrated physical mapping of human chromosome 22 using BACs. Annual report, July 1994--June 1995

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BACs and fosmids are stable, nonchimeric, and highly representative cloning systems. BACs maintain large-fragment genomic inserts (100 to 300 kb) that are easily prepared for most types of experiments, including DNA sequencing. The authors have improved the methods for generating BACs and developed extensive BAC libraries. They have constructed human BAC libraries with more than 175,000 clones from male fibroblast and sperm, and a mouse BAC library with more than 200,000 clones. The authors are currently expanding human library with the aim of achieving total 50X coverage human genomic library using sperm samples from anonymous donors.

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1 p.

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Kim, U.J.; Shizuya, Hiroaki & Simon, M.I. December 31, 1995.

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Description

BACs and fosmids are stable, nonchimeric, and highly representative cloning systems. BACs maintain large-fragment genomic inserts (100 to 300 kb) that are easily prepared for most types of experiments, including DNA sequencing. The authors have improved the methods for generating BACs and developed extensive BAC libraries. They have constructed human BAC libraries with more than 175,000 clones from male fibroblast and sperm, and a mouse BAC library with more than 200,000 clones. The authors are currently expanding human library with the aim of achieving total 50X coverage human genomic library using sperm samples from anonymous donors.

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1 p.

Notes

OSTI as DE98007130

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  • Other Information: PBD: [1995]

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  • Other: DE98007130
  • Report No.: DOE/ER/60891--T6
  • Grant Number: FG03-89ER60891
  • DOI: 10.2172/639707 | External Link
  • Office of Scientific & Technical Information Report Number: 639707
  • Archival Resource Key: ark:/67531/metadc689794

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  • December 31, 1995

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  • Aug. 14, 2015, 8:43 a.m.

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  • Nov. 4, 2015, 4:20 p.m.

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Kim, U.J.; Shizuya, Hiroaki & Simon, M.I. Development of BAC libraries and integrated physical mapping of human chromosome 22 using BACs. Annual report, July 1994--June 1995, report, December 31, 1995; United States. (digital.library.unt.edu/ark:/67531/metadc689794/: accessed September 22, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.