Optical Detection of Aqueous Phase Analytes via Host-Guest Interactions on a Lipid Membrane Surface

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Description

The organization and assembly of molecules in cellular membranes is orchestrated through the recognition and binding of specific chemical signals. A simplified version of the cellular membrane system has been developed using a synthetically prepared membrane receptor incorporated into a biologically derived lipid bilayer. Through an interplay of electrostatic and van der Wards interactions, aggregation or dispersion of molecular components could be executed on command using a specific chemical signal. A pyrene fluorophore was used as an optical probe to monitor the aggregational state of the membrane receptors in the bilayer matrix. The pyrene excimer emission to monomer emission (E/M) ... continued below

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11 p.

Creation Information

Sasaki, D.Y. & Waggoner, T.A. January 11, 1999.

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This article is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. More information about this article can be viewed below.

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  • Sandia National Laboratories
    Publisher Info: Sandia National Labs., Albuquerque, NM, and Livermore, CA (United States)
    Place of Publication: Albuquerque, New Mexico

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Description

The organization and assembly of molecules in cellular membranes is orchestrated through the recognition and binding of specific chemical signals. A simplified version of the cellular membrane system has been developed using a synthetically prepared membrane receptor incorporated into a biologically derived lipid bilayer. Through an interplay of electrostatic and van der Wards interactions, aggregation or dispersion of molecular components could be executed on command using a specific chemical signal. A pyrene fluorophore was used as an optical probe to monitor the aggregational state of the membrane receptors in the bilayer matrix. The pyrene excimer emission to monomer emission (E/M) intensity ratio gave a relative assessment of the local concentration of receptors in the membrane. Bilayers were prepared with receptors selective for the divalent metal ions of copper, mercury, and lead. Addition of the metal ions produced a rapid dispersion of aggregated receptor components at nano- to micro-molar concentrations. The process was reversible by sequestering the metal ions with EDTA. Receptors for proteins and polyhistidine were also prepared and incorporated into phosphatidylcholine lipid bilayers. In this case, the guest molecules bound to the membrane through multiple points of interaction causing aggregation of initially dispersed receptor molecules. The rapid, selective, and sensitive fluorescence optical response of these lipid assemblies make them attractive in sensor applications for aqueous phase metal ions and polypeptides.

Physical Description

11 p.

Notes

OSTI as DE00003254

Medium: P; Size: 11 pages

Source

  • Photonics West '99 - BiOS '99 International Biomedical Optics Symposium, San Jose, CA (US), 01/22/1999--01/29/1999

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  • Report No.: SAND99-0083C
  • Grant Number: AC04-94AL85000
  • Office of Scientific & Technical Information Report Number: 3254
  • Archival Resource Key: ark:/67531/metadc688973

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  • January 11, 1999

Added to The UNT Digital Library

  • July 25, 2015, 2:20 a.m.

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  • April 6, 2017, 7:32 p.m.

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Sasaki, D.Y. & Waggoner, T.A. Optical Detection of Aqueous Phase Analytes via Host-Guest Interactions on a Lipid Membrane Surface, article, January 11, 1999; Albuquerque, New Mexico. (digital.library.unt.edu/ark:/67531/metadc688973/: accessed September 19, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.