Capillary electrophoretic study of individual exocytotic events in single mast cells

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The peak profile of individual degranulation events from the on-column release of serotonin from single rat peritoneal mast cells (RPMCs) was monitored using capillary electrophoresis with laser-induced native fluorescence detection (CE-LINF). Serotonin, an important biogenic amine, is contained in granules (0.25 fL) within RPMCs and is extruded by a process termed exocytosis. The secretagogue, Polymyxin B sulfate, was used as the CE running buffer after injection of a single RPMC into the separation capillary to stimulate the release of the granules. Because the release process occurs on a ms time scale, monitoring individual exocytotic events is possible with the coupling ... continued below

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14 p.

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Ho, A.M.W. February 12, 1999.

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This thesis or dissertation is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. More information about this document can be viewed below.

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  • Ames Laboratory
    Publisher Info: Ames Lab., IA (United States)
    Place of Publication: Iowa

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Description

The peak profile of individual degranulation events from the on-column release of serotonin from single rat peritoneal mast cells (RPMCs) was monitored using capillary electrophoresis with laser-induced native fluorescence detection (CE-LINF). Serotonin, an important biogenic amine, is contained in granules (0.25 fL) within RPMCs and is extruded by a process termed exocytosis. The secretagogue, Polymyxin B sulfate, was used as the CE running buffer after injection of a single RPMC into the separation capillary to stimulate the release of the granules. Because the release process occurs on a ms time scale, monitoring individual exocytotic events is possible with the coupling of high-speed CE and LINF detection.

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14 p.

Notes

OSTI as DE99002525

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  • Other Information: DN: Thesis submitted to Iowa State Univ., Ames, IA (US); TH: Thesis (M.S.)

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Identifier

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  • Other: DE99002525
  • Report No.: IS-T--1860
  • Grant Number: W-7405-ENG-82
  • Office of Scientific & Technical Information Report Number: 348903
  • Archival Resource Key: ark:/67531/metadc688369

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  • February 12, 1999

Added to The UNT Digital Library

  • July 25, 2015, 2:20 a.m.

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  • Nov. 6, 2015, 8:52 p.m.

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Ho, A.M.W. Capillary electrophoretic study of individual exocytotic events in single mast cells, thesis or dissertation, February 12, 1999; Iowa. (digital.library.unt.edu/ark:/67531/metadc688369/: accessed September 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.