Effects of diesel exhaust on the microbiota within a tuffaceous tunnel system Metadata

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  • Main Title Effects of diesel exhaust on the microbiota within a tuffaceous tunnel system


  • Author: Haldeman, D.L.
    Creator Type: Personal
  • Author: Lagadinos, T.
    Creator Type: Personal
  • Author: Amy, P.S.
    Creator Type: Personal
    Creator Info: Univ. of Nevada, Las Vegas, NV (United States)
  • Author: Hersman, L.
    Creator Type: Personal
    Creator Info: Los Alamos National Lab., NM (United States)
  • Author: Meike, A.
    Creator Type: Personal
    Creator Info: Lawrence Livermore National Lab., Livermore, CA (United States)


  • Sponsor: United States. Department of Energy. Office of Civilian Radioactive Waste Management.
    Contributor Type: Organization
    Contributor Info: USDOE Office of Civilian Radioactive Waste Management, Washington, DC (United States)


  • Name: Lawrence Livermore National Laboratory
    Place of Publication: California
    Additional Info: Lawrence Livermore National Lab., CA (United States)


  • Creation: 1996-08-01


  • English


  • Content Description: The abundance and distribution of microbiota that may be impacted by diesel and diesel exhaust were investigated from three depths into the walls and invert (floor) of U12n tunnel at Rainier Mesa, Nevada Test Site, a potential geological analog of Yucca Mountain. Enumerations included total cell counts, and numbers of aerobic heterotrophic, sulfate-reducing, nitrate-reducing, and diesel-degrading bacteria. Additionally, the disappearance of total petroleum hydrocarbons was determined in microcosms containing subsurface materials that were amended with diesel fuel. Results revealed that microbes capable of utilizing diesel and diesel combustion products were present in the subsurface in both the walls and the invert of the tunnel. The abundance of specific bacterial types in the tunnel invert, a perturbed environment, was greater than that observed in the tunnel wall. Few trends of microbial distribution either into the tunnel wall or the invert were noted with the exception of aerobic heterotrophic abundance which increased with depth into the wall and decreased with depth into the invert. No correlation between microbiota and a specific introduced chemical species have yet been determined. The potential for microbial contamination of the tunnel wall during sampling was determined to be negligible by the use of fluorescently labeled latex spheres (1{mu}m in dia.) as tracers. Results indicate that additional investigations might be needed to examine the microbiota and their possible impacts on the geology and geochemistry of the subsurface, both indigenous microbiota and those microorganisms that will likely be introduced by anthropogenic activity associated with the construction of a high-level waste repository.
  • Physical Description: 37 p.


  • Keyword: Hydrocarbons
  • Keyword: Combustion
  • Keyword: Exhaust Gases
  • Keyword: Diesel Fuels
  • Keyword: Microcosms
  • Keyword: Combustion Products
  • STI Subject Categories: 05 Nuclear Fuels
  • STI Subject Categories: 55 Biology And Medicine, Basic Studies
  • Keyword: Underground Facilities
  • Keyword: Geochemistry
  • STI Subject Categories: 02 Petroleum
  • Keyword: Performance
  • Keyword: Underground Disposal
  • Keyword: High-Level Radioactive Wastes
  • Keyword: Petroleum
  • Keyword: Bacteria
  • Keyword: Biological Effects
  • Keyword: Yucca Mountain


  • Other Information: PBD: Aug 1996


  • Name: Office of Scientific & Technical Information Technical Reports
    Code: OSTI


  • Name: UNT Libraries Government Documents Department
    Code: UNTGD

Resource Type

  • Report


  • Text


  • Other: DE97050753
  • Report No.: UCRL-ID--125176
  • Grant Number: W-7405-ENG-48
  • DOI: 10.2172/420416
  • Office of Scientific & Technical Information Report Number: 420416
  • Archival Resource Key: ark:/67531/metadc685147


  • Display Note: INIS; OSTI as DE97050753