An integrated multiple capillary array electrophoresis system for high-throughput DNA sequencing

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Description

A capillary array electrophoresis system was chosen to perform DNA sequencing because of several advantages such as rapid heat dissipation, multiplexing capabilities, gel matrix filling simplicity, and the mature nature of the associated manufacturing technologies. There are two major concerns for the multiple capillary systems. One concern is inter-capillary cross-talk, and the other concern is excitation and detection efficiency. Cross-talk is eliminated through proper optical coupling, good focusing and immersing capillary array into index matching fluid. A side-entry excitation scheme with orthogonal detection was established for large capillary array. Two 100 capillary array formats were used for DNA sequencing. One ... continued below

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158 p.

Creation Information

Lu, X. March 27, 1998.

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This report is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. More information about this report can be viewed below.

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  • Ames Laboratory
    Publisher Info: Ames Lab., IA (United States)
    Place of Publication: Iowa

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Description

A capillary array electrophoresis system was chosen to perform DNA sequencing because of several advantages such as rapid heat dissipation, multiplexing capabilities, gel matrix filling simplicity, and the mature nature of the associated manufacturing technologies. There are two major concerns for the multiple capillary systems. One concern is inter-capillary cross-talk, and the other concern is excitation and detection efficiency. Cross-talk is eliminated through proper optical coupling, good focusing and immersing capillary array into index matching fluid. A side-entry excitation scheme with orthogonal detection was established for large capillary array. Two 100 capillary array formats were used for DNA sequencing. One format is cylindrical capillary with 150 {micro}m o.d., 75 {micro}m i.d and the other format is square capillary with 300 {micro}m out edge and 75 {micro}m inner edge. This project is focused on the development of excitation and detection of DNA as well as performing DNA sequencing. The DNA injection schemes are discussed for the cases of single and bundled capillaries. An individual sampling device was designed. The base-calling was performed for a capillary from the capillary array with the accuracy of 98%.

Physical Description

158 p.

Notes

OSTI as DE99002505

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  • Other Information: DN: Thesis submitted to Iowa State Univ., Ames, IA (US); TH: Thesis (Ph.D.)

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  • Other: DE99002505
  • Report No.: IS-T--1830
  • Grant Number: W-7405-ENG-82
  • DOI: 10.2172/348901 | External Link
  • Office of Scientific & Technical Information Report Number: 348901
  • Archival Resource Key: ark:/67531/metadc684896

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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Creation Date

  • March 27, 1998

Added to The UNT Digital Library

  • July 25, 2015, 2:20 a.m.

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  • Nov. 6, 2015, 8:46 p.m.

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Lu, X. An integrated multiple capillary array electrophoresis system for high-throughput DNA sequencing, report, March 27, 1998; Iowa. (digital.library.unt.edu/ark:/67531/metadc684896/: accessed October 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.