Actinide biocolloid formation in brine by halophilic bacteria

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The authors examined the ability of a halophilic bacterium (WIPP 1A) isolated from the Waste Isolation Pilot Plant (WIPP) site to accumulate uranium in order to determine the potential for biocolloid facilitated actinide transport. The bacterial cell surface functional groups involved in the complexation of the actinide were determined by titration. Uranium, added as uranyl nitrate, was removed from solution at pH 5 by cells but at pH 7 and 9 very little uranium was removed due to its limited solubility. Although present as soluble species, uranyl citrate at pH 5, 7, and 9, and uranyl carbonate at pH 9 ... continued below

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8 p.

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Gillow, J.B.; Francis, A.J.; Dodge, C.J.; Harris, R.; Beveridge, T.J.; Brady, P.V. et al. December 31, 1998.

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Description

The authors examined the ability of a halophilic bacterium (WIPP 1A) isolated from the Waste Isolation Pilot Plant (WIPP) site to accumulate uranium in order to determine the potential for biocolloid facilitated actinide transport. The bacterial cell surface functional groups involved in the complexation of the actinide were determined by titration. Uranium, added as uranyl nitrate, was removed from solution at pH 5 by cells but at pH 7 and 9 very little uranium was removed due to its limited solubility. Although present as soluble species, uranyl citrate at pH 5, 7, and 9, and uranyl carbonate at pH 9 were not removed by the bacterium because they were not bioavailable due to their neutral or negative charge. Addition of uranyl EDTA to brine at pH 5, 7, and 9 resulted in the immediate precipitation of U. Transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDS) analysis revealed that uranium was not only associated with the cell surface but also accumulated intracellularly as uranium-enriched granules. Extended X-ray absorption fine structure (EXAFS) analysis of the bacterial cells indicated the bulk sample contained more than one uranium phase. Nevertheless these results show the potential for the formation of actinide bearing bacterial biocolloids that are strictly regulated by the speciation and bioavailability of the actinide.

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8 p.

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INIS; OSTI as DE99001519

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  • 22. scientific basis for nuclear waste management, Boston, MA (United States), 30 Nov - 4 Dec 1998

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  • Other: DE99001519
  • Report No.: BNL--66064
  • Report No.: CONF-981132--
  • Grant Number: AC02-98CH10886
  • DOI: 10.2172/307836 | External Link
  • Office of Scientific & Technical Information Report Number: 307836
  • Archival Resource Key: ark:/67531/metadc684721

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Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • December 31, 1998

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  • July 25, 2015, 2:20 a.m.

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  • Nov. 10, 2015, 1:20 p.m.

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Gillow, J.B.; Francis, A.J.; Dodge, C.J.; Harris, R.; Beveridge, T.J.; Brady, P.V. et al. Actinide biocolloid formation in brine by halophilic bacteria, report, December 31, 1998; Upton, New York. (digital.library.unt.edu/ark:/67531/metadc684721/: accessed October 24, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.