The addition of a second lanthanide ion to increase the luminescence of europium(III) macrocyclic complexes

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At present, the microscopic visualization of luminescent labels containing lanthanide(III) ions, primarily europium(III), as light-emitting centers is best performed with time-gated instrumentation, which by virtually eliminating the background fluorescence results in an improved signal to noise ratio. However, the use of the europium(III) macrocycle, Quantum Dye{trademark}, in conjunction with the strong luminescence enhancing effect (cofluorescence) of yttrium(III) or gadolinium(III), can eliminate the need for such specialized instrumentation. In the presence of Gd(III), the luminescence of the Eu(III)-macrocycles can be conveniently observed with conventional fluorescence instrumentation at previously unattainable low levels. The Eu(III) {sup 5}D{sub 0} {r_arrow} {sup 7}F{sub 2} emission ... continued below

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11 p.

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Bromm, A.J. Jr.; Vallarino, L.M.; Leif, R.C. & Quagliano, J.R. December 29, 1998.

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Description

At present, the microscopic visualization of luminescent labels containing lanthanide(III) ions, primarily europium(III), as light-emitting centers is best performed with time-gated instrumentation, which by virtually eliminating the background fluorescence results in an improved signal to noise ratio. However, the use of the europium(III) macrocycle, Quantum Dye{trademark}, in conjunction with the strong luminescence enhancing effect (cofluorescence) of yttrium(III) or gadolinium(III), can eliminate the need for such specialized instrumentation. In the presence of Gd(III), the luminescence of the Eu(III)-macrocycles can be conveniently observed with conventional fluorescence instrumentation at previously unattainable low levels. The Eu(III) {sup 5}D{sub 0} {r_arrow} {sup 7}F{sub 2} emission of the Eu(III)-macrocycles was observed as an extremely sharp band with a maximum at 619 nm and a clearly resolved characteristic pattern. At very low Eu(III)-macrocycle concentrations, another sharp emission was detected at 614 nm, arising from traces of Eu(III) present in even the purest commercially available gadolinium products. Discrimination of the resolved emissions of the Eu(III)-macrocycle and Eu(III) contaminant should provide a means to further lower the limit of detection of the Eu(III)-macrocycle.

Physical Description

11 p.

Notes

OSTI as DE99001752

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  • Photonics West `99: international symposium on biomedical optics (BIOS`99), San Jose, CA (United States), 23-29 Jan 1999

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  • Other: DE99001752
  • Report No.: LA-UR--99-61
  • Report No.: CONF-990110--
  • Grant Number: W-7405-ENG-36
  • DOI: 10.2172/314151 | External Link
  • Office of Scientific & Technical Information Report Number: 314151
  • Archival Resource Key: ark:/67531/metadc681216

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  • December 29, 1998

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  • July 25, 2015, 2:20 a.m.

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  • Feb. 25, 2016, 1:12 p.m.

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Bromm, A.J. Jr.; Vallarino, L.M.; Leif, R.C. & Quagliano, J.R. The addition of a second lanthanide ion to increase the luminescence of europium(III) macrocyclic complexes, report, December 29, 1998; New Mexico. (digital.library.unt.edu/ark:/67531/metadc681216/: accessed August 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.