Analysis of the function of the agouti gene in obesity and diabetes

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This chapter discusses the agouti gene and dominant mutations in that gene that lead to agouti-induced obesity, and recent work with transgenic mice to elucidate the role of agouti in obesity. Agouti was cloned in 1992 by the lab of Rick Woychik at Oak Ridge National Laboratory, making it the first of many recently cloned mouse obesity genes. Sequence analysis predicted that mouse agouti is a secreted protein of 131 amino acids. The mature protein has a basic central region (lys57-arg85), a proline-rich domain (pro86-pro91) and a C-terminal region (cys 92-cys 13 1) containing 10 cysteine residues which form 5 ... continued below

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15 p.

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Mynatt, R.L.; Miltenberger, R.J. & Klebig, M.L. September 1, 1996.

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This article is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 13 times , with 10 in the last month . More information about this article can be viewed below.

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Description

This chapter discusses the agouti gene and dominant mutations in that gene that lead to agouti-induced obesity, and recent work with transgenic mice to elucidate the role of agouti in obesity. Agouti was cloned in 1992 by the lab of Rick Woychik at Oak Ridge National Laboratory, making it the first of many recently cloned mouse obesity genes. Sequence analysis predicted that mouse agouti is a secreted protein of 131 amino acids. The mature protein has a basic central region (lys57-arg85), a proline-rich domain (pro86-pro91) and a C-terminal region (cys 92-cys 13 1) containing 10 cysteine residues which form 5 disulfide bonds. The human homologue of agouti has also been cloned by the Woychik lab and maps to human chromosome 20q 11.2. Human agouti is 132 amino acids long and is 85% similar to the mouse agouti protein and is normally expressed in adipose tissue. The researchers have been able to recapitulate obesity, hyperinsulinemia, and hyperglycemia with the ubiquitous expression of agouti. Agouti expression in either liver and adipose tissue alone does not cause obesity, and there`s a dose-dependent effect of agouti on body weight, food efficiency, body temperature, and insulin and glucose levels.

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15 p.

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OSTI as DE96015028

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  • 2. annual international symposium on obesity, Washington, DC (United States), 4-6 Mar 1996

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  • Other: DE96015028
  • Report No.: CONF-9603200--1
  • Grant Number: AC05-96OR22464
  • Office of Scientific & Technical Information Report Number: 376394
  • Archival Resource Key: ark:/67531/metadc680661

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  • September 1, 1996

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  • July 25, 2015, 2:20 a.m.

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  • Jan. 21, 2016, 3:24 p.m.

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Mynatt, R.L.; Miltenberger, R.J. & Klebig, M.L. Analysis of the function of the agouti gene in obesity and diabetes, article, September 1, 1996; Tennessee. (digital.library.unt.edu/ark:/67531/metadc680661/: accessed September 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.