Biocatalytic removal of organic sulfur from coal

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Description

The objective is to characterize more completely the biochemical ability of the bacterium, Rhodococcus rhodochrous IGTS8, to cleave carbon-sulfur bonds with emphasis on data that will allow the development of a practical coal biodesulfurization process. Another approach for increasing the desulfurization activity of the IGTS8 cultures is to produce strains genetically that have higher activity. The goal of this part of research is to achieve strain improvement by introducing a stronger promoter using genetic engineering techniques. The promoter regulates the transcription of the genes for the desulfurization enzymes, and a stronger promoter, would up-regulate the expression of these genes, resulting ... continued below

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15 p.

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Webster, D.A. & Kilbane, J.J. II September 9, 1994.

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Description

The objective is to characterize more completely the biochemical ability of the bacterium, Rhodococcus rhodochrous IGTS8, to cleave carbon-sulfur bonds with emphasis on data that will allow the development of a practical coal biodesulfurization process. Another approach for increasing the desulfurization activity of the IGTS8 cultures is to produce strains genetically that have higher activity. The goal of this part of research is to achieve strain improvement by introducing a stronger promoter using genetic engineering techniques. The promoter regulates the transcription of the genes for the desulfurization enzymes, and a stronger promoter, would up-regulate the expression of these genes, resulting in cells with higher desulfurization activity. Promoter probe vectors are used to identify and isolate promoters from a DNA library of the experimental organism. The major accomplishments have been to obtain high biodesulfurization activity in nonaqueous, media, especially using freeze-dried cells, and to have isolated strong promoters from R. rhodochrous IGTS8 which will be used to engineer the organism to produce strains with higher biocatalytic activity.

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15 p.

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OSTI as DE95009349

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  • Other Information: PBD: 9 Sep 1994

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  • Other: DE95009349
  • Report No.: DOE/PC/92549--T8
  • Grant Number: FG22-92PC92549
  • DOI: 10.2172/36801 | External Link
  • Office of Scientific & Technical Information Report Number: 36801
  • Archival Resource Key: ark:/67531/metadc679548

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  • September 9, 1994

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  • July 25, 2015, 2:20 a.m.

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  • Nov. 18, 2015, 6:48 p.m.

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Webster, D.A. & Kilbane, J.J. II. Biocatalytic removal of organic sulfur from coal, report, September 9, 1994; United States. (digital.library.unt.edu/ark:/67531/metadc679548/: accessed September 22, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.