Ionizing radiation-induced DNA damage and its repair in human cells. Final performance report, July 1992--June 1995

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Description

The studies of DNA damage in living cells in vitro and in vivo were continued. A variety of systems including cultured mammalian cells, animals, and human tissues were used to conduct these studies. In addition, enzymatic repair of DNA base damage was studied using several DNA glycosylases. To this end, substrate specificities of these enzymes were examined in terms of a large number of base lesions in DNA. In the first phase of the studies, the author sought to introduce improvements to his methodologies for measurement of DNA damage using the technique of gas chromatography/mass spectrometry (GC/MS). In particular, the ... continued below

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[35] p.

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Dizdaroglu, M. December 31, 1995.

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Description

The studies of DNA damage in living cells in vitro and in vivo were continued. A variety of systems including cultured mammalian cells, animals, and human tissues were used to conduct these studies. In addition, enzymatic repair of DNA base damage was studied using several DNA glycosylases. To this end, substrate specificities of these enzymes were examined in terms of a large number of base lesions in DNA. In the first phase of the studies, the author sought to introduce improvements to his methodologies for measurement of DNA damage using the technique of gas chromatography/mass spectrometry (GC/MS). In particular, the quantitative measurement of DNA base damage and DNA-protein crosslinks was improved by incorporation of isotope-dilution mass spectrometry into the methodologies. This is one of the most accurate techniques for quantification of organic compounds. Having improved the measurement technique, studies of DNA damage in living cells and DNA repair by repair enzymes were pursued. This report provides a summary of these studies with references to the original work.

Physical Description

[35] p.

Notes

INIS; OSTI as DE99002798

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  • Other Information: PBD: [1995]

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  • Other: DE99002798
  • Report No.: DOE/ER/60826--T2
  • Grant Number: AI05-89ER60826
  • DOI: 10.2172/353368 | External Link
  • Office of Scientific & Technical Information Report Number: 353368
  • Archival Resource Key: ark:/67531/metadc679378

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  • December 31, 1995

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  • July 25, 2015, 2:20 a.m.

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  • Nov. 5, 2015, 12:19 p.m.

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Dizdaroglu, M. Ionizing radiation-induced DNA damage and its repair in human cells. Final performance report, July 1992--June 1995, report, December 31, 1995; United States. (digital.library.unt.edu/ark:/67531/metadc679378/: accessed September 19, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.