Coupling sequencing by hybridization (SBH) with gel sequencing for an inexpensive analysis of genes and genomes

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Description

The speed and cost of DNA sequencing are bottlenecks in the analysis of genes end genomes. Sequencing by hybridization (SBH) is a versatile method with several applications which can accelerated DNA screening, mapping and sequencing. Requirements, achievements and problems in the development of the SBH format 1 (DNA samples arrayed) are presented and schemes for its synergetic coupling with gel sequencing techniques are discussed. It appears that by one hybridization machine with 24 boxes and four ABI gel sequencers 100- 300 Mb of DNA sequence can be determined per year. Various genetic studies based on computer assisted analysis of large ... continued below

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36 p.

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Drmanac, S.; Labat, I.; Hauser, B. & Drmanac, R. November 1, 1996.

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Description

The speed and cost of DNA sequencing are bottlenecks in the analysis of genes end genomes. Sequencing by hybridization (SBH) is a versatile method with several applications which can accelerated DNA screening, mapping and sequencing. Requirements, achievements and problems in the development of the SBH format 1 (DNA samples arrayed) are presented and schemes for its synergetic coupling with gel sequencing techniques are discussed. It appears that by one hybridization machine with 24 boxes and four ABI gel sequencers 100- 300 Mb of DNA sequence can be determined per year. Various genetic studies based on computer assisted analysis of large collections of partial or complete DNA sequences (`sequenetics`) may be achieved in this century.

Physical Description

36 p.

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INIS; OSTI as DE97001019

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  • Other Information: PBD: [1996]

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  • Other: DE97001019
  • Report No.: ANL/CMB/PP--83848
  • Grant Number: W-31109-ENG-38
  • DOI: 10.2172/405751 | External Link
  • Office of Scientific & Technical Information Report Number: 405751
  • Archival Resource Key: ark:/67531/metadc677861

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  • November 1, 1996

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  • July 25, 2015, 2:20 a.m.

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  • Dec. 16, 2015, 12:14 p.m.

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Drmanac, S.; Labat, I.; Hauser, B. & Drmanac, R. Coupling sequencing by hybridization (SBH) with gel sequencing for an inexpensive analysis of genes and genomes, report, November 1, 1996; Illinois. (digital.library.unt.edu/ark:/67531/metadc677861/: accessed September 25, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.