New fluorescence methodology for detecting DNA adducts. Final progress report, May 1, 1991--November 30, 1994

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Description

A new reagent, {open_quotes}BO-IMI{close_quotes}, has been developed that achieves, for the first time, single step, phosphate specific fluorescence labeling under aqueous conditions. Both 3{prime} and 5{prime} mononucleotides, including representative DNA adducts can be labeled. Included in this technique is a convenient procedure for postlabeling sample cleanup, leading to a practical detection of the products by capillary electrophoresis with laser fluorescence detection (CE-LIF). We consider that this new method will have a significant impact on the measurement of DNA adducts in human samples. This work was largely accomplished in the second half of our project. In the first half, we set ... continued below

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7 p.

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Giese, R.W. December 19, 1994.

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Description

A new reagent, {open_quotes}BO-IMI{close_quotes}, has been developed that achieves, for the first time, single step, phosphate specific fluorescence labeling under aqueous conditions. Both 3{prime} and 5{prime} mononucleotides, including representative DNA adducts can be labeled. Included in this technique is a convenient procedure for postlabeling sample cleanup, leading to a practical detection of the products by capillary electrophoresis with laser fluorescence detection (CE-LIF). We consider that this new method will have a significant impact on the measurement of DNA adducts in human samples. This work was largely accomplished in the second half of our project. In the first half, we set up a new way to isolate DNA nucleotides from blood, worked with an initial, less specific technique for labeling DNA adducts, compared ionizing radiation vs oxidative damage to fluorescein labeled deoxyadenylic acid, and set up a capillary electrophoresis laser fluorescence detection system.

Physical Description

7 p.

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OSTI as DE95006408

Medium: P; Size: 7 p.

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  • Other Information: PBD: 19 Dec 1994

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  • Other: DE95006408
  • Report No.: DOE/ER/60964--T1
  • Grant Number: FG02-90ER60964
  • DOI: 10.2172/29367 | External Link
  • Office of Scientific & Technical Information Report Number: 29367
  • Archival Resource Key: ark:/67531/metadc675882

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  • December 19, 1994

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  • July 25, 2015, 2:20 a.m.

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  • April 13, 2017, 12:48 p.m.

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Giese, R.W. New fluorescence methodology for detecting DNA adducts. Final progress report, May 1, 1991--November 30, 1994, report, December 19, 1994; United States. (digital.library.unt.edu/ark:/67531/metadc675882/: accessed September 25, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.