Development of an expression system for eukarytoic proteins in methylotropic bacteria Metadata
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Title
- Main Title Development of an expression system for eukarytoic proteins in methylotropic bacteria
Creator
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Author: Lidstrom, M.E.Creator Type: PersonalCreator Info: California Inst. of Tech., Pasadena, CA (United States)
Contributor
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Sponsor: United States. Department of Energy.Contributor Type: OrganizationContributor Info: USDOE, Washington, DC (United States)
Publisher
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Name: Los Alamos National LaboratoryPlace of Publication: New MexicoAdditional Info: Los Alamos National Lab., NM (United States)
Date
- Creation: 1996-09-01
Language
- English
Description
- Content Description: The objective of this project was to develop an expression vector for methylotrophic bacteria for use in the production of C{sup 13} and H{sup 2} labelled eukaryotic proteins by growing methylotrophic bacteria on labelled methanol or methylamine. The eukaryotic proteins calmodulin and troponin C were chosen as test cases. Genes encoding both proteins were cloned into different constructions and tested for expression. Moderate amounts of troponin C were found with one of the constructions.
- Physical Description: 3 p.
Subject
- Keyword: Protein Engineering
- Keyword: Calmodulin
- Keyword: Dna-Cloning
- Keyword: Methylamine
- Keyword: Labelled Compounds
- Keyword: Genetic Engineering
- Keyword: Progress Report
- STI Subject Categories: 55 Biology And Medicine, Basic Studies
- Keyword: Production
- Keyword: Methanol
- Keyword: Bacteria
Source
- Other Information: PBD: [1996]
Collection
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Name: Office of Scientific & Technical Information Technical ReportsCode: OSTI
Institution
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Name: UNT Libraries Government Documents DepartmentCode: UNTGD
Resource Type
- Report
Format
- Text
Identifier
- Other: DE96015159
- Report No.: LA-SUB--96-103
- Grant Number: W-7405-ENG-36
- DOI: 10.2172/378670
- Office of Scientific & Technical Information Report Number: 378670
- Archival Resource Key: ark:/67531/metadc675212
Note
- Display Note: OSTI as DE96015159