Development of an expression system for eukarytoic proteins in methylotropic bacteria Metadata

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Title

  • Main Title Development of an expression system for eukarytoic proteins in methylotropic bacteria

Creator

  • Author: Lidstrom, M.E.
    Creator Type: Personal
    Creator Info: California Inst. of Tech., Pasadena, CA (United States)

Contributor

  • Sponsor: United States. Department of Energy.
    Contributor Type: Organization
    Contributor Info: USDOE, Washington, DC (United States)

Publisher

  • Name: Los Alamos National Laboratory
    Place of Publication: New Mexico
    Additional Info: Los Alamos National Lab., NM (United States)

Date

  • Creation: 1996-09-01

Language

  • English

Description

  • Content Description: The objective of this project was to develop an expression vector for methylotrophic bacteria for use in the production of C{sup 13} and H{sup 2} labelled eukaryotic proteins by growing methylotrophic bacteria on labelled methanol or methylamine. The eukaryotic proteins calmodulin and troponin C were chosen as test cases. Genes encoding both proteins were cloned into different constructions and tested for expression. Moderate amounts of troponin C were found with one of the constructions.
  • Physical Description: 3 p.

Subject

  • Keyword: Protein Engineering
  • Keyword: Calmodulin
  • Keyword: Dna-Cloning
  • Keyword: Methylamine
  • Keyword: Labelled Compounds
  • Keyword: Genetic Engineering
  • Keyword: Progress Report
  • STI Subject Categories: 55 Biology And Medicine, Basic Studies
  • Keyword: Production
  • Keyword: Methanol
  • Keyword: Bacteria

Source

  • Other Information: PBD: [1996]

Collection

  • Name: Office of Scientific & Technical Information Technical Reports
    Code: OSTI

Institution

  • Name: UNT Libraries Government Documents Department
    Code: UNTGD

Resource Type

  • Report

Format

  • Text

Identifier

  • Other: DE96015159
  • Report No.: LA-SUB--96-103
  • Grant Number: W-7405-ENG-36
  • DOI: 10.2172/378670
  • Office of Scientific & Technical Information Report Number: 378670
  • Archival Resource Key: ark:/67531/metadc675212

Note

  • Display Note: OSTI as DE96015159