Factors limiting microbial activity in volcanic tuff at Yucca Mountain

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Description

Samples of tuff aseptically collected from 10 locations in the Exploratory Shaft Facility at the site of the proposed high-level nuclear waste repository at Yucca Mountain, Nevada Test Site were analyzed for microbiological populations, activities, and factors limiting microbial activity. Radiotracer assays ({sup 14}C-labeled organic substrate mineralization), direct microscopic counts, and plate counts were used. Radiolabeled substrates were glucose, acetate, and glutamate. Radiotracer experiments were carried out with and without moisture and inorganic nutrient amendments to determine factors limiting to microbial activities. Nearly all samples showed the presence of microorganisms with the potential to mineralize organic substrates. Addition of inorganic ... continued below

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4 p.

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Kieft, T.L.; Kovacik, W.P. & Taylor, J. September 1, 1996.

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Description

Samples of tuff aseptically collected from 10 locations in the Exploratory Shaft Facility at the site of the proposed high-level nuclear waste repository at Yucca Mountain, Nevada Test Site were analyzed for microbiological populations, activities, and factors limiting microbial activity. Radiotracer assays ({sup 14}C-labeled organic substrate mineralization), direct microscopic counts, and plate counts were used. Radiolabeled substrates were glucose, acetate, and glutamate. Radiotracer experiments were carried out with and without moisture and inorganic nutrient amendments to determine factors limiting to microbial activities. Nearly all samples showed the presence of microorganisms with the potential to mineralize organic substrates. Addition of inorganic nutrients stimulated activities in a small number of samples. The presence of viable microbial communities within the tuff has implications for transport of contaminants.

Physical Description

4 p.

Notes

INIS; OSTI as DE96015158

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  • Other Information: PBD: [1996]

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  • Other: DE96015158
  • Report No.: LA-SUB--96-105
  • Grant Number: W-7405-ENG-36
  • DOI: 10.2172/379091 | External Link
  • Office of Scientific & Technical Information Report Number: 379091
  • Archival Resource Key: ark:/67531/metadc674788

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  • September 1, 1996

Added to The UNT Digital Library

  • July 25, 2015, 2:20 a.m.

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  • July 28, 2016, 7:14 p.m.

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Kieft, T.L.; Kovacik, W.P. & Taylor, J. Factors limiting microbial activity in volcanic tuff at Yucca Mountain, report, September 1, 1996; New Mexico. (digital.library.unt.edu/ark:/67531/metadc674788/: accessed December 12, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.