Enzymology of acetoclastic methanogenesis. Final technical report, August 1994--July 1997

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The overall aims of the proposal were to identify intermediates in the process of methane formation from acetate. The specific aims were: (1) to characterize the mechanism of acetyl-CoA conversion to CH{sub 3}-H{sub 4}MPT, CO, and CoA and elucidate the mechanism of CO oxidation by the methanosarcina thermophila CO dehydrogenase/acetyl-CoA synthase (CODH/ACS), and (2) to study the mechanism of methyl-CoM reduction to methane by developing an activation protocol for methyl-CoM reductase (MCR) and identifying intermediates in the MCR mechanism. The authors also made significant progress toward accomplishing two other specific aims that were not included in the original proposal. These ... continued below

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7 p.

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Ragsdale, S.W. December 31, 1997.

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Description

The overall aims of the proposal were to identify intermediates in the process of methane formation from acetate. The specific aims were: (1) to characterize the mechanism of acetyl-CoA conversion to CH{sub 3}-H{sub 4}MPT, CO, and CoA and elucidate the mechanism of CO oxidation by the methanosarcina thermophila CO dehydrogenase/acetyl-CoA synthase (CODH/ACS), and (2) to study the mechanism of methyl-CoM reduction to methane by developing an activation protocol for methyl-CoM reductase (MCR) and identifying intermediates in the MCR mechanism. The authors also made significant progress toward accomplishing two other specific aims that were not included in the original proposal. These were: (1) to characterize the M. thermophila heterodisulfide reductase (HDR), and (2) to characterize the methyltetrahydromethanopterin: Coenzyme M methyltransferase from Methanosarcina mazei strain Goe1 in collaboration with Gerhard Gottschalk`s group. Results from these four studies are summarized.

Physical Description

7 p.

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OSTI as DE99002028

Medium: P; Size: 7 p.

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  • Other Information: PBD: [1997]

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  • Other: DE99002028
  • Report No.: DOE/ER/20053--T1
  • Grant Number: FG02-91ER20053
  • DOI: 10.2172/325726 | External Link
  • Office of Scientific & Technical Information Report Number: 325726
  • Archival Resource Key: ark:/67531/metadc674705

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  • December 31, 1997

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  • July 25, 2015, 2:20 a.m.

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  • April 13, 2017, 12:47 p.m.

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Ragsdale, S.W. Enzymology of acetoclastic methanogenesis. Final technical report, August 1994--July 1997, report, December 31, 1997; United States. (digital.library.unt.edu/ark:/67531/metadc674705/: accessed September 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.