Identification of cDNA clones encoding valosin-containing protein and other plant plasma membrane-associated proteins by a general immunoscreening strategy

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Article on the identification of cDNA clones encoding valosin-containing protein and other plant plasma membrane-associated proteins by a general immunoscreening strategy.

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5 p.

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Shi, Jinrui; Dixon, R. A.; Gonzales, Robert A.; Kjellbom, Per & Bhattacharyya, Madan Kumar May 1, 1995.

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Article on the identification of cDNA clones encoding valosin-containing protein and other plant plasma membrane-associated proteins by a general immunoscreening strategy.

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5 p.

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Abstract: An approach was developed for the isolation and characterization of soybean plasma membrane-associated proteins by immunoscreening of a cDNA expression library. An antiserum was raised against purified plasma membrane vesicles. In a differential screening of approximately 500,000 plaque-forming units with the anti-(plasma membrane) serum and DNA probes derived from highly abundant clones isolated in a preliminary screening, 261 clones were selected from approximately 1,200 antiserum-positive plaques. These clones were classified into 40 groups by hybridization analysis and 5'- and 3'-terminal sequencing. By searching nucleic acid and protein sequence data bases, 11 groups of cDNAs were identified, among which valosin-containing protein (VCP), clathrin heavy chain, phospholipase C, and S-adenosylmethionine:delta 24-sterol-C-methyltransferase have not to date been cloned from plants. The remaining 29 groups did not match any current data base entries and may, therefore, represent additional or yet uncharacterized genes. A full-length cDNA encoding the soybean VCP was sequenced. The high level of amino acid identity with vertebrate VCP and yeast CDC48 protein indicates that the soybean protein is a plant homolog of vertebrate VCP and yeast CDC48 protein.

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  • Proceedings of the National Academy of Sciences (U.S.), 1995, Washington D.C.: National Academy of Sciences (U.S.), pp. 4457-4461

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  • Publication Title: Proceedings of the National Academy of Sciences (U.S.)
  • Volume: 92
  • Issue: 10
  • Page Start: 4457
  • Page End: 4461
  • Pages: 5
  • Peer Reviewed: Yes

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  • May 1, 1995

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  • July 9, 2015, 6:19 a.m.

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Shi, Jinrui; Dixon, R. A.; Gonzales, Robert A.; Kjellbom, Per & Bhattacharyya, Madan Kumar. Identification of cDNA clones encoding valosin-containing protein and other plant plasma membrane-associated proteins by a general immunoscreening strategy, article, May 1, 1995; [Washington, D.C.]. (digital.library.unt.edu/ark:/67531/metadc674047/: accessed January 18, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.