Membrane boenergetics of salt tolerant organisms. Progress report, June 1993--June 1995

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Substantial progress was made on describing the pathway of the transported proton in bacteriorhodopsin, and the thermodynamics of the proton transfers. The underlying principle of the transport was identified as the alternating access of the retinal Schiff base toward the two membrane surfaces, regulated by electrostatic interaction between the retinylidene nitrogen and its counterion. Consistent with a shared transport mechanism for both retinal proteins, bacteriorhodopsin was converted into a balorhodopsin-like chloride pump by replacing asp-85 with threonine. This region is thereby identified as the active site that determines ion specificity. Description of the metal ion-dependent kinetics of the ATP hydrolysis ... continued below

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7 p.

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Lanyi, J.K. June 1, 1996.

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Description

Substantial progress was made on describing the pathway of the transported proton in bacteriorhodopsin, and the thermodynamics of the proton transfers. The underlying principle of the transport was identified as the alternating access of the retinal Schiff base toward the two membrane surfaces, regulated by electrostatic interaction between the retinylidene nitrogen and its counterion. Consistent with a shared transport mechanism for both retinal proteins, bacteriorhodopsin was converted into a balorhodopsin-like chloride pump by replacing asp-85 with threonine. This region is thereby identified as the active site that determines ion specificity. Description of the metal ion-dependent kinetics of the ATP hydrolysis provided clues to the structure of active site in the halobacterial ATPase.

Physical Description

7 p.

Notes

OSTI as DE96011177

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  • Other Information: PBD: [1996]

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  • Other: DE96011177
  • Report No.: DOE/ER/13525--T3
  • Grant Number: FG03-86ER13525
  • DOI: 10.2172/239341 | External Link
  • Office of Scientific & Technical Information Report Number: 239341
  • Archival Resource Key: ark:/67531/metadc673014

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  • June 1, 1996

Added to The UNT Digital Library

  • June 29, 2015, 9:42 p.m.

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  • Nov. 24, 2015, 12:14 p.m.

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Lanyi, J.K. Membrane boenergetics of salt tolerant organisms. Progress report, June 1993--June 1995, report, June 1, 1996; United States. (digital.library.unt.edu/ark:/67531/metadc673014/: accessed July 16, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.