The effects of 5-fluorouracil and doxorubicin on expression of human immunodeficiency virus type 1 long terminal repeat

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Description

Previous work by many groups has documented induction of the HIV-LTR following exposure of cells to ultraviolet light and other DNA damaging agents. Our experiments set out to determine the relative activation or repression of the HIV-LTR in response to two classes of chemotherapeutic agents: Doxorubicin is a DNA-damage inducing agent, and 5-fluorouracil has an antimetabolic mode of action. Using HeLa cells stably transfected with a construct in which HIV-LTR drives expression of the chloramphenicol acetyl transferase reporter gene, we demonstrated an up to 10-fold induction following doxorubicin treatment in 24 h post-treatment. This induction was repressed by treatment with ... continued below

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25 p.

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Panozzo, J.; Akan, E.; Griffiths, T. D. & Woloschak, G. E. March 1996.

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Description

Previous work by many groups has documented induction of the HIV-LTR following exposure of cells to ultraviolet light and other DNA damaging agents. Our experiments set out to determine the relative activation or repression of the HIV-LTR in response to two classes of chemotherapeutic agents: Doxorubicin is a DNA-damage inducing agent, and 5-fluorouracil has an antimetabolic mode of action. Using HeLa cells stably transfected with a construct in which HIV-LTR drives expression of the chloramphenicol acetyl transferase reporter gene, we demonstrated an up to 10-fold induction following doxorubicin treatment in 24 h post-treatment. This induction was repressed by treatment with salicylic acid, suggesting a role for prostaglandin/cyclo-oxygenase pathways and/or NFKB in the inductive response. Induction by 5-fluorouracil, in contrast, was more modest (two-fold at most) though it was consistently elevated over controls.

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25 p.

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INIS; OSTI as DE96007466

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  • Other Information: PBD: [1996]

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  • Other: DE96007466
  • Report No.: ANL/CMB/PP--86653
  • Grant Number: W-31109-ENG-38
  • DOI: 10.2172/207377 | External Link
  • Office of Scientific & Technical Information Report Number: 207377
  • Archival Resource Key: ark:/67531/metadc672872

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  • March 1996

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  • June 29, 2015, 9:42 p.m.

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  • Dec. 16, 2015, 12:24 p.m.

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Panozzo, J.; Akan, E.; Griffiths, T. D. & Woloschak, G. E. The effects of 5-fluorouracil and doxorubicin on expression of human immunodeficiency virus type 1 long terminal repeat, report, March 1996; Illinois. (digital.library.unt.edu/ark:/67531/metadc672872/: accessed September 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.