Testing of thiol-terminated oligonucleotides as potential DNA-cleaving reagents

PDF Version Also Available for Download.

Description

In this study the authors have conducted experiments to determine if oligonucleotides containing terminal thiol groups can be used, in the presence of Cu+2, to cut single- and double-stranded DNA molecules adjacent to the oligonucleotide binding site. The results of these experiments demonstrate that these modified oligonucleotides cleave DNA in the presence of Cu+2, but the reaction could not be controlled sufficiently to attain site-selective cleavage or minimize degradation of the thiol terminated oligonucleotide.

Physical Description

13 p.

Creation Information

Balhorn, R. & Mazrimas, J. June 1, 1996.

Context

This report is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 17 times . More information about this report can be viewed below.

Who

People and organizations associated with either the creation of this report or its content.

Authors

  • Balhorn, R.
  • Mazrimas, J. Lawrence Livermore National Lab., CA (United States). Biology and Biotechnology Research Program

Sponsor

Publisher

Provided By

UNT Libraries Government Documents Department

Serving as both a federal and a state depository library, the UNT Libraries Government Documents Department maintains millions of items in a variety of formats. The department is a member of the FDLP Content Partnerships Program and an Affiliated Archive of the National Archives.

Contact Us

What

Descriptive information to help identify this report. Follow the links below to find similar items on the Digital Library.

Description

In this study the authors have conducted experiments to determine if oligonucleotides containing terminal thiol groups can be used, in the presence of Cu+2, to cut single- and double-stranded DNA molecules adjacent to the oligonucleotide binding site. The results of these experiments demonstrate that these modified oligonucleotides cleave DNA in the presence of Cu+2, but the reaction could not be controlled sufficiently to attain site-selective cleavage or minimize degradation of the thiol terminated oligonucleotide.

Physical Description

13 p.

Notes

OSTI as DE96013201

Source

  • Other Information: PBD: Jun 1996

Language

Item Type

Identifier

Unique identifying numbers for this report in the Digital Library or other systems.

  • Other: DE96013201
  • Report No.: UCRL-ID--124126
  • Grant Number: W-7405-ENG-48
  • DOI: 10.2172/270720 | External Link
  • Office of Scientific & Technical Information Report Number: 270720
  • Archival Resource Key: ark:/67531/metadc672503

Collections

This report is part of the following collection of related materials.

Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

What responsibilities do I have when using this report?

When

Dates and time periods associated with this report.

Creation Date

  • June 1, 1996

Added to The UNT Digital Library

  • June 29, 2015, 9:42 p.m.

Description Last Updated

  • Feb. 23, 2016, 12:39 p.m.

Usage Statistics

When was this report last used?

Yesterday: 0
Past 30 days: 1
Total Uses: 17

Interact With This Report

Here are some suggestions for what to do next.

Start Reading

PDF Version Also Available for Download.

Citations, Rights, Re-Use

Balhorn, R. & Mazrimas, J. Testing of thiol-terminated oligonucleotides as potential DNA-cleaving reagents, report, June 1, 1996; California. (digital.library.unt.edu/ark:/67531/metadc672503/: accessed November 17, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.