Protecting drinking water: Rapid detection of human fecal contamination, injured and non-culturable pathogenic microbes in water systems

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The rapid, potentially-automatable extraction of filter retentates has allowed quantitative detection of the unique biomarker for human fecal contamination, coprostanol, and the signature lipid biomarkers for total cellular biomass, viable cellular biomass, lipopolysaccharide (endotoxin). This method may be integrated with DNA based gene probe analysis for specific strains and enzyme activities. Not only does the analysis provide for detection of injured and non-culturable microbes but it also provides biomarkers characteristic of microbes exposed to biocides and disinfectants that can be utilized to monitor effectiveness of water mitigation/treatment. The analysis schemes involve filtration of the water or direct extraction of biofilms ... continued below

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7 p.

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White, D.C.; Nivens, D.E.; Arrage, A.A.; Appelgate, B.M.; Reardon, S.R. & Sayler, G.S. May 1, 1996.

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Description

The rapid, potentially-automatable extraction of filter retentates has allowed quantitative detection of the unique biomarker for human fecal contamination, coprostanol, and the signature lipid biomarkers for total cellular biomass, viable cellular biomass, lipopolysaccharide (endotoxin). This method may be integrated with DNA based gene probe analysis for specific strains and enzyme activities. Not only does the analysis provide for detection of injured and non-culturable microbes but it also provides biomarkers characteristic of microbes exposed to biocides and disinfectants that can be utilized to monitor effectiveness of water mitigation/treatment. The analysis schemes involve filtration of the water or direct extraction of biofilms in sidestream chambers, supercritical fluid and/or liquid extraction, derivatization, and analysis of ``signature`` patterns by gas chromatography/mass spectrometry. Signature lipid biomarkers of interest are diglycerides, steroids including coprostanol and its isomers, poly-{beta}- hydroxyalcanoates (PHA), phospholipid ester-linked fatty acids (PLFA), and the lipopolysaccharide lipid A hydroxy fatty acids. PLFA found in polar lipid fractions estimate total viable cellular biomass, whereas the total cellular biomass can be calculated from diglyceride/phospholipid ester-linked fatty acids ratios. Furthermore, direct evidence of mitigation/treatment effectiveness can be ascertained by detection of diglycerides, respiratory quinones, PHA, and PLFA markers indicative of metabolic stress and toxicity such as trans monoenoic PLFA as well as oxirane and dicarboxylic fatty acids derived from the PLFA.

Physical Description

7 p.

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OSTI as DE96009889

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  • 1996 North America water and environment congress, Anaheim, CA (United States), 27-28 Jun 1996

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  • Other: DE96009889
  • Report No.: CONF-9606154--1
  • Grant Number: AC05-96OR22464
  • Office of Scientific & Technical Information Report Number: 242690
  • Archival Resource Key: ark:/67531/metadc670828

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • May 1, 1996

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  • June 29, 2015, 9:42 p.m.

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  • Jan. 25, 2016, 2:11 p.m.

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White, D.C.; Nivens, D.E.; Arrage, A.A.; Appelgate, B.M.; Reardon, S.R. & Sayler, G.S. Protecting drinking water: Rapid detection of human fecal contamination, injured and non-culturable pathogenic microbes in water systems, article, May 1, 1996; Tennessee. (digital.library.unt.edu/ark:/67531/metadc670828/: accessed October 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.