Laser mass spectrometry for DNA fingerprinting for forensic applications

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The application of DNA fingerprinting has become very broad in forensic analysis, patient identification, diagnostic medicine, and wildlife poaching, since every individual`s DNA structure is identical within all tissues of their body. DNA fingerprinting was initiated by the use of restriction fragment length polymorphisms (RFLP). In 1987, Nakamura et al. found that a variable number of tandem repeats (VNTR) often occurred in the alleles. The probability of different individuals having the same number of tandem repeats in several different alleles is very low. Thus, the identification of VNTR from genomic DNA became a very reliable method for identification of individuals. ... continued below

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10 p.

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Chen, C.H.; Tang, K.; Taranenko, N.I.; Allman, S.L. & Chang, L.Y. December 31, 1994.

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Description

The application of DNA fingerprinting has become very broad in forensic analysis, patient identification, diagnostic medicine, and wildlife poaching, since every individual`s DNA structure is identical within all tissues of their body. DNA fingerprinting was initiated by the use of restriction fragment length polymorphisms (RFLP). In 1987, Nakamura et al. found that a variable number of tandem repeats (VNTR) often occurred in the alleles. The probability of different individuals having the same number of tandem repeats in several different alleles is very low. Thus, the identification of VNTR from genomic DNA became a very reliable method for identification of individuals. DNA fingerprinting is a reliable tool for forensic analysis. In DNA fingerprinting, knowledge of the sequence of tandem repeats and restriction endonuclease sites can provide the basis for identification. The major steps for conventional DNA fingerprinting include (1) specimen processing (2) amplification of selected DNA segments by PCR, and (3) gel electrophoresis to do the final DNA analysis. In this work we propose to use laser desorption mass spectrometry for fast DNA fingerprinting. The process and advantages are discussed.

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10 p.

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OSTI as DE96009390

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  • Annual meeting of the Society of Photo-Optical Instrumentation Engineers, San Diego, CA (United States), 24-29 Jul 1994

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  • Other: DE96009390
  • Report No.: CONF-940723--45
  • Grant Number: AC05-84OR21400
  • Office of Scientific & Technical Information Report Number: 230358
  • Archival Resource Key: ark:/67531/metadc666308

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  • December 31, 1994

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  • June 29, 2015, 9:42 p.m.

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  • Jan. 25, 2016, 2:15 p.m.

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Chen, C.H.; Tang, K.; Taranenko, N.I.; Allman, S.L. & Chang, L.Y. Laser mass spectrometry for DNA fingerprinting for forensic applications, article, December 31, 1994; Tennessee. (digital.library.unt.edu/ark:/67531/metadc666308/: accessed September 24, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.