Catalytic activity of nuclease P1: Experiment and theory Metadata

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  • Main Title Catalytic activity of nuclease P1: Experiment and theory


  • Author: Miller, J.H.
    Creator Type: Personal
    Creator Info: Pacific Northwest Lab., Richland, WA (United States)
  • Author: Falcone, J.M.
    Creator Type: Personal
  • Author: Shibata, M.
    Creator Type: Personal
  • Author: Box, H.C.
    Creator Type: Personal
    Creator Info: Roswell Park Cancer Institute, Buffalo, New York (United States)


  • Sponsor: United States. Department of Energy.
    Contributor Type: Organization
    Contributor Info: USDOE, Washington, DC (United States)


  • Name: Pacific Northwest Laboratory
    Place of Publication: Richland, Washington
    Additional Info: Pacific Northwest Lab., Richland, WA (United States)


  • Creation: 1994-10-01


  • English


  • Content Description: Nuclease P1 from Penicillium citrinum is a zinc dependent glyco-enzyme that recognizes single stranded DNA and RNA as substrates and hydrolyzes the phosphate ester bond. Nuclease Pl seems to recognize particular conformations of the phosphodiester backbone and shows significant variation in the rate of hydrolytic activity depending upon which nucleosides are coupled by the phosphodiester bond. The efficiency of nuclease Pl in hydrolyzing the phosphodiester bonds of a substrate can be altered by modifications to one of the substrate bases induced by ionizing radiation or oxidative stress. Measurements have been made of the effect of several radiation induced lesions on the catalytic rate of nuclease Pl. A model of the structure of the enzyme has been constructed in order to better understand the binding and activity of this enzyme on various ssDNA substrates.
  • Physical Description: 9 p.


  • Keyword: Protein Structure
  • Keyword: Nucleases
  • Keyword: Fungi
  • STI Subject Categories: 55 Biology And Medicine, Basic Studies
  • Keyword: Radiation Induced Mutants
  • Keyword: Gene Mutations
  • STI Subject Categories: 56 Biology And Medicine, Applied Studies
  • Keyword: Biochemical Reaction Kinetics


  • Conference: International workshop on radiation damage in DNA: relationships at early times, Gleneden, OR (United States), 1-6 Oct 1994


  • Name: Office of Scientific & Technical Information Technical Reports
    Code: OSTI


  • Name: UNT Libraries Government Documents Department
    Code: UNTGD

Resource Type

  • Article


  • Text


  • Other: DE95004956
  • Report No.: PNL-SA--25462
  • Report No.: CONF-9410280--1
  • Grant Number: AC06-76RL01830
  • Office of Scientific & Technical Information Report Number: 26690
  • Archival Resource Key: ark:/67531/metadc666014


  • Display Note: INIS; OSTI as DE95004956