Structural determination of larger proteins using stable isotope labeling and NMR spectroscopy

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Description

The project sought to employ stable isotope labeling and NMR spectroscopy to study protein structures and provide insight into important biochemical problems. A methylotrophic bacterial expression system has been developed for uniform deuterium and carbon-13 labeling of proteins for structural studies. These organisms grow using methanol as the sole source of carbon and energy. Because isotopically labeled methanol is relatively inexpensive, the methylotrophs are ideal for expressing proteins labeled uniformly with deuterium and/or carbon-13. This expression system has been employed to prepare deuterated troponin C. NMR spectroscopy measurements have been made on the inhibitory peptide from troponin I (residues 96--115), ... continued below

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5 p.

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Unkefer, C.; Hernandez, G.; Springer, P.; Trewhella, J.; Blumenthal, D. & Lidstrom, M. April 1, 1996.

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Description

The project sought to employ stable isotope labeling and NMR spectroscopy to study protein structures and provide insight into important biochemical problems. A methylotrophic bacterial expression system has been developed for uniform deuterium and carbon-13 labeling of proteins for structural studies. These organisms grow using methanol as the sole source of carbon and energy. Because isotopically labeled methanol is relatively inexpensive, the methylotrophs are ideal for expressing proteins labeled uniformly with deuterium and/or carbon-13. This expression system has been employed to prepare deuterated troponin C. NMR spectroscopy measurements have been made on the inhibitory peptide from troponin I (residues 96--115), both as the free peptide and the peptide complexed with deuterated troponin C. Proton-NMR spectroscopy resonance-signal assignments have been made for the free peptide.

Physical Description

5 p.

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OSTI as DE96008775

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  • Other Information: PBD: [1996]

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  • Other: DE96008775
  • Report No.: LA-UR--96-0239
  • Grant Number: W-7405-ENG-36
  • DOI: 10.2172/212499 | External Link
  • Office of Scientific & Technical Information Report Number: 212499
  • Archival Resource Key: ark:/67531/metadc665944

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Creation Date

  • April 1, 1996

Added to The UNT Digital Library

  • June 29, 2015, 9:42 p.m.

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  • Feb. 26, 2016, 6:27 p.m.

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Unkefer, C.; Hernandez, G.; Springer, P.; Trewhella, J.; Blumenthal, D. & Lidstrom, M. Structural determination of larger proteins using stable isotope labeling and NMR spectroscopy, report, April 1, 1996; New Mexico. (digital.library.unt.edu/ark:/67531/metadc665944/: accessed August 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.