Near-infrared spectroscopy of lysed blood: pH effects

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Recent investigations by our group have demonstrated that near-infrared spectra collected from lysed blood solutions can be used to create clinically useful partial least squares (PSL) models for pH with standard errors of prediction below 0.05 pH units for pH range of 1 (6.8 to 7.8). Further work was performed in order to discern the primary source of pH information in the spectra. Results from these experiments are presented using spectral data acquired over the spectral range of 1300 nm to 2500 nm from plasma, lysed blood and amino acids solutions. Data were analyzed by principal component analysis (PCA) and ... continued below

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10 p.

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Alam, M. K.; Franke, J. E.; Maynard, J. D.; Robinson, M. R.; Niemczyk, T. M.; Rohrschieb, M. et al. December 1995.

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  • Sandia National Laboratories
    Publisher Info: Sandia National Labs., Albuquerque, NM (United States)
    Place of Publication: Albuquerque, New Mexico

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Description

Recent investigations by our group have demonstrated that near-infrared spectra collected from lysed blood solutions can be used to create clinically useful partial least squares (PSL) models for pH with standard errors of prediction below 0.05 pH units for pH range of 1 (6.8 to 7.8). Further work was performed in order to discern the primary source of pH information in the spectra. Results from these experiments are presented using spectral data acquired over the spectral range of 1300 nm to 2500 nm from plasma, lysed blood and amino acids solutions. Data were analyzed by principal component analysis (PCA) and loading vectors were compared. Experiments were designed to eliminate possible correlation between pH and other components in the system in order to ensure variations in the spectral data were due to hydrogen ion changes only. Results indicate that variations in the spectral characteristics of histidine mimic those seen in lysed blood, but not those seen in plasma, suggesting that histidine residues from hemoglobin are providing the necessary variation for pH modeling in the lysed blood solutions.

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10 p.

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OSTI as DE96006382

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  • Photonics West `96: conference on quantum well and superlattice physics VI, San Jose, CA (United States), 27 Jan - 2 Feb 1996

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  • Other: DE96006382
  • Report No.: SAND--95-1926C
  • Report No.: CONF-960163--11
  • Grant Number: AC04-94AL85000
  • Office of Scientific & Technical Information Report Number: 207378
  • Archival Resource Key: ark:/67531/metadc665732

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  • December 1995

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  • June 29, 2015, 9:42 p.m.

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  • Dec. 9, 2015, 1:49 p.m.

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Alam, M. K.; Franke, J. E.; Maynard, J. D.; Robinson, M. R.; Niemczyk, T. M.; Rohrschieb, M. et al. Near-infrared spectroscopy of lysed blood: pH effects, article, December 1995; Albuquerque, New Mexico. (digital.library.unt.edu/ark:/67531/metadc665732/: accessed November 19, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.