Chemical Cleavage of Human Phosphoglucose Isomerase at Cysteine Metadata
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- Main Title Chemical Cleavage of Human Phosphoglucose Isomerase at Cysteine
Author: Conn, Worth R.Creator Type: Personal
Chair: Gracy, Robert W.Contributor Type: PersonalContributor Info: Major Professor
Committee Member: Russell, BennyContributor Type: PersonalContributor Info: Minor Professor
Committee Member: Jacobson, MyronContributor Type: Personal
Name: North Texas State UniversityPlace of Publication: Denton, Texas
- Creation: 1975-12
- Content Description: The present study has resulted in the development of a procedure for the specific chemical fragmentation of human phosphoglucose isomerase into a minimal number of peptides. A two-cycle procedure for cleaving the protein with 2-nitro-5- thiocyanobenzoic acid results in four primary peptides and three overlap peptides. The peptides can be readily separated on the basis of their size by using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Preliminary peptide alignments have been considered, and amino acid analyses have been performed. End-terminal analyses of the enzyme revealed a carboxyl terminal sequence of Asp-Val-Gln and a blocked amino terminus. The cysteine cleavage procedure provides an excellent method for the identification and location of specific genetic mutations of human phosphoglucose isomerase.
- Physical Description: 69 leaves: ill.
- Keyword: phosphoglucose isomerase
- Library of Congress Subject Headings: Phosphoglucose isomerase -- Analysis.
- Keyword: cysteine cleavage procedure
Name: UNT Theses and DissertationsCode: UNTETD
Name: UNT LibrariesCode: UNT
- Rights Access: public
- Rights Holder: Conn, Worth R.
- Rights License: copyright
- Rights Statement: Copyright is held by the author, unless otherwise noted. All rights
- Thesis or Dissertation
- Accession or Local Control No: 1002773322-Conn
- Call Number: 379 N81 no.5085
- UNT Catalog No.: b1153930
- OCLC: 2168923
- Archival Resource Key: ark:/67531/metadc663679
- Academic Department: Department of Chemistry
- Degree Discipline: Chemistry
- Degree Level: Master's
- Degree Name: Master of Science
- Degree Publication Type: thesi
- Degree Grantor: University of North Texas