Chemical Cleavage of Human Phosphoglucose Isomerase at Cysteine Metadata

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Title

  • Main Title Chemical Cleavage of Human Phosphoglucose Isomerase at Cysteine

Creator

  • Author: Conn, Worth R.
    Creator Type: Personal

Contributor

  • Chair: Gracy, Robert W.
    Contributor Type: Personal
    Contributor Info: Major Professor
  • Committee Member: Russell, Benny
    Contributor Type: Personal
    Contributor Info: Minor Professor
  • Committee Member: Jacobson, Myron
    Contributor Type: Personal

Publisher

  • Name: North Texas State University
    Place of Publication: Denton, Texas

Date

  • Creation: 1975-12

Language

  • English

Description

  • Content Description: The present study has resulted in the development of a procedure for the specific chemical fragmentation of human phosphoglucose isomerase into a minimal number of peptides. A two-cycle procedure for cleaving the protein with 2-nitro-5- thiocyanobenzoic acid results in four primary peptides and three overlap peptides. The peptides can be readily separated on the basis of their size by using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Preliminary peptide alignments have been considered, and amino acid analyses have been performed. End-terminal analyses of the enzyme revealed a carboxyl terminal sequence of Asp-Val-Gln and a blocked amino terminus. The cysteine cleavage procedure provides an excellent method for the identification and location of specific genetic mutations of human phosphoglucose isomerase.
  • Physical Description: 69 leaves: ill.

Subject

  • Keyword: phosphoglucose isomerase
  • Library of Congress Subject Headings: Phosphoglucose isomerase -- Analysis.
  • Keyword: cysteine cleavage procedure

Collection

  • Name: UNT Theses and Dissertations
    Code: UNTETD

Institution

  • Name: UNT Libraries
    Code: UNT

Rights

  • Rights Access: public
  • Rights Holder: Conn, Worth R.
  • Rights License: copyright
  • Rights Statement: Copyright is held by the author, unless otherwise noted. All rights

Resource Type

  • Thesis or Dissertation

Format

  • Text

Identifier

  • Accession or Local Control No: 1002773322-Conn
  • Call Number: 379 N81 no.5085
  • UNT Catalog No.: b1153930
  • OCLC: 2168923
  • Archival Resource Key: ark:/67531/metadc663679

Degree

  • Academic Department: Department of Chemistry
  • Degree Discipline: Chemistry
  • Degree Level: Master's
  • Degree Name: Master of Science
  • Degree Publication Type: thesi
  • Degree Grantor: University of North Texas

Note