Denaturation, Renaturation and Other Structural Studies on Phosphoglucose Isomerases

Thumbnail image of item number 1 in: 'Denaturation, Renaturation and Other Structural Studies on Phosphoglucose Isomerases'.
Thumbnail image of item number 2 in: 'Denaturation, Renaturation and Other Structural Studies on Phosphoglucose Isomerases'.
Thumbnail image of item number 3 in: 'Denaturation, Renaturation and Other Structural Studies on Phosphoglucose Isomerases'.
Thumbnail image of item number 4 in: 'Denaturation, Renaturation and Other Structural Studies on Phosphoglucose Isomerases'.
Showing 1-4 of 67 pages in this thesis.

PDF Version Also Available for Download.

Description

Structural properties of phosphoglucose isomerases isolated from a variety of species have been compared by peptide fingerprinting, predicted amino acid sequence homologies and by denaturation and renaturation studies. The enzymes are more readily denatured in guanidinium chloride than in urea, and the isomerase isolated from yeast is more stable toward acid pH than the rabbit muscle enzyme. The rates of guanidinium chloride-induced denaturation are markedly increased by ionic strength and decreased by substrates, competitive inhibitors or glycerol. The enzyme can be renatured, but only in the presence of glycerol. The renaturation process is dependent on protein concentration and temperature and ... continued below

Physical Description

65 leaves: ill.

Creation Information

Young, Clint D. December 1975.

Context

This thesis is part of the collection entitled: UNT Theses and Dissertations and was provided by UNT Libraries to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 16 times . More information about this thesis can be viewed below.

Who

People and organizations associated with either the creation of this thesis or its content.

Author

Chair

Committee Members

Publisher

Rights Holder

For guidance see Citations, Rights, Re-Use.

  • Young, Clint D.

Provided By

UNT Libraries

The UNT Libraries serve the university and community by providing access to physical and online collections, fostering information literacy, supporting academic research, and much, much more.

About | Browse this Partner

Contact Us

Corrections Questions

What

Descriptive information to help identify this thesis. Follow the links below to find similar items on the Digital Library.

Degree Information

Description

Structural properties of phosphoglucose isomerases isolated from a variety of species have been compared by peptide fingerprinting, predicted amino acid sequence homologies and by denaturation and renaturation studies. The enzymes are more readily denatured in guanidinium chloride than in urea, and the isomerase isolated from yeast is more stable toward acid pH than the rabbit muscle enzyme. The rates of guanidinium chloride-induced denaturation are markedly increased by ionic strength and decreased by substrates, competitive inhibitors or glycerol. The enzyme can be renatured, but only in the presence of glycerol. The renaturation process is dependent on protein concentration and temperature and provides a method for the formation of mixed species heterodimers.

Physical Description

65 leaves: ill.

Language

Item Type

Identifier

Unique identifying numbers for this thesis in the Digital Library or other systems.

Collections

This thesis is part of the following collection of related materials.

UNT Theses and Dissertations

Theses and dissertations represent a wealth of scholarly and artistic content created by masters and doctoral students in the degree-seeking process. Some ETDs in this collection are restricted to use by the UNT community.

About | Browse this Collection

What responsibilities do I have when using this thesis?

Digital Files

When

Dates and time periods associated with this thesis.

Creation Date

  • December 1975

Added to The UNT Digital Library

  • June 24, 2015, 9:39 a.m.

Description Last Updated

  • July 25, 2016, 10:50 a.m.

Usage Statistics

When was this thesis last used?

Yesterday: 0
Past 30 days: 0
Total Uses: 16
More Statistics

Interact With This Thesis

Here are some suggestions for what to do next.

Start Reading

Thumbnail image of item number 1 in: 'Denaturation, Renaturation and Other Structural Studies on Phosphoglucose Isomerases'.
Thumbnail image of item number 2 in: 'Denaturation, Renaturation and Other Structural Studies on Phosphoglucose Isomerases'.
Thumbnail image of item number 3 in: 'Denaturation, Renaturation and Other Structural Studies on Phosphoglucose Isomerases'.
Thumbnail image of item number 4 in: 'Denaturation, Renaturation and Other Structural Studies on Phosphoglucose Isomerases'.

PDF Version Also Available for Download.

Citations, Rights, Re-Use

International Image Interoperability Framework

IIF Logo

We support the IIIF Presentation API

Young, Clint D. Denaturation, Renaturation and Other Structural Studies on Phosphoglucose Isomerases, thesis, December 1975; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc663054/: accessed October 22, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .