Advanced microinstrumentation for rapid DNA sequencing and large DNA fragment separation

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Description

Our efforts to develop novel technology for a rapid DNA sequencer and large fragment analysis system based upon gel electrophoresis are described. We are using microfabrication technology to build dense arrays of high speed micro electrophoresis lanes that will ultimately increase the sequencing rate of DNA by at least 100 times the rate of current sequencers. We have demonstrated high resolution DNA fragment separation needed for sequencing in polyacrylamide microgels formed in glass microchannels. We have built prototype arrays of microchannels having up to 48 channels. Significant progress has also been made in developing a sensitive fluorescence detection system based ... continued below

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12 p.

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Balch, J.; Davidson, J.; Brewer, L.; Gingrich, J.; Koo, J.; Mariella, R. et al. January 25, 1995.

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Description

Our efforts to develop novel technology for a rapid DNA sequencer and large fragment analysis system based upon gel electrophoresis are described. We are using microfabrication technology to build dense arrays of high speed micro electrophoresis lanes that will ultimately increase the sequencing rate of DNA by at least 100 times the rate of current sequencers. We have demonstrated high resolution DNA fragment separation needed for sequencing in polyacrylamide microgels formed in glass microchannels. We have built prototype arrays of microchannels having up to 48 channels. Significant progress has also been made in developing a sensitive fluorescence detection system based upon a confocal microscope design that will enable the diagnostics and detection of DNA fragments in ultrathin microchannel gels. Development of a rapid DNA sequencer and fragment analysis system will have a major impact on future DNA instrumentation used in clinical, molecular and forensic analysis of DNA fragments.

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12 p.

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OSTI as DE95017042

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  • Other Information: PBD: 25 Jan 1995

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  • Other: DE95017042
  • Report No.: UCRL-ID--119660
  • Grant Number: W-7405-ENG-48
  • DOI: 10.2172/105702 | External Link
  • Office of Scientific & Technical Information Report Number: 105702
  • Archival Resource Key: ark:/67531/metadc626660

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Office of Scientific & Technical Information Technical Reports

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  • January 25, 1995

Added to The UNT Digital Library

  • June 16, 2015, 7:43 a.m.

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  • Feb. 18, 2016, 11:28 a.m.

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Balch, J.; Davidson, J.; Brewer, L.; Gingrich, J.; Koo, J.; Mariella, R. et al. Advanced microinstrumentation for rapid DNA sequencing and large DNA fragment separation, report, January 25, 1995; California. (digital.library.unt.edu/ark:/67531/metadc626660/: accessed September 26, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.