Laser desorption mass spectrometry for fast DNA analysis

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During the past few years, major effort has been directed toward developing mass spectrometry to measure biopolymers because of the great potential benefit to biomedical research. Hellenkamp and his co-workers were the first to report that large polypeptide molecules can be ionized and detected without significant fragmentation when a greater number of nicotinic acid molecules are used as a matrix. This method is now well known as matrix-assisted laser desorption/ionization (MALDI). Since then, various groups have reported measurements of very large proteins by MALDI. Reliable protein analysis by MALDI is more or less well established. However, the application of MALDI ... continued below

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9 p.

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Chen, C.H.; Ch`ang, L.Y.; Taranenko, N.I.; Allman, S.L.; Tang, K. & Matteson, K.J. September 1995.

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Description

During the past few years, major effort has been directed toward developing mass spectrometry to measure biopolymers because of the great potential benefit to biomedical research. Hellenkamp and his co-workers were the first to report that large polypeptide molecules can be ionized and detected without significant fragmentation when a greater number of nicotinic acid molecules are used as a matrix. This method is now well known as matrix-assisted laser desorption/ionization (MALDI). Since then, various groups have reported measurements of very large proteins by MALDI. Reliable protein analysis by MALDI is more or less well established. However, the application of MALDI to nucleic acids analysis has been found to be much more difficult. Most research on the measurement of nucleic acid by MALDI were stimulated by the Human Genome Project. Up to now, the only method for reliable routine analysis of nucleic acid is gel electrophoresis. Different sizes of nucleic acids can be separated in gel medium when a high electric field is applied to the gel. However, the time needed to separate different sizes of DNA segments usually takes from several minutes to several hours. If MALDI can be successfully used for nucleic acids analysis, the analysis time can be reduced to less than I millisecond. In addition, no tagging with radioactive materials or chemical dyes is needed. In this work, we will review recent progress related to MALDI for DNA analysis.

Physical Description

9 p.

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OSTI as DE95017392

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  • Chinese-American Academic and Professional Association in United States (CAPASUS) conference, Atlanta, GA (United States), 23-25 Jun 1995

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  • Other: DE95017392
  • Report No.: CONF-9506258--1
  • Grant Number: AC05-84OR21400
  • Office of Scientific & Technical Information Report Number: 108175
  • Archival Resource Key: ark:/67531/metadc626596

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  • September 1995

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  • June 16, 2015, 7:43 a.m.

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  • May 2, 2016, 3:55 p.m.

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Chen, C.H.; Ch`ang, L.Y.; Taranenko, N.I.; Allman, S.L.; Tang, K. & Matteson, K.J. Laser desorption mass spectrometry for fast DNA analysis, article, September 1995; Tennessee. (digital.library.unt.edu/ark:/67531/metadc626596/: accessed September 24, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.