c-jun gene expression in human cells exposed to either ionizing radiation or hydrogen peroxide

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We investigated the role of reactive oxygen intermediates (ROIs) and protein kinase C (PKC) in radiation- and H{sub 2}O{sub 2}-evoked c-jun gene expression in human HL-205 cells. This induction of c-jun gene expression could be prevented by pretreatment of the cells with Nacetylcysteine (an antioxidant) or H7 (a PKC and PKA inhibitor) but not by HA1004, a PKA inhibitor, suggesting a role for ROls and PKC in mediating c-jun gene expression. We also investigated potential differences in c-jun gene expression in a panel of normal and tumor cells untreated or treated with ionizing radiation or H{sub 2}O{sub 2}. Treatment with ... continued below

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6 p.

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Collart, F.R.; Horio, M. & Huberman, E. June 1, 1993.

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We investigated the role of reactive oxygen intermediates (ROIs) and protein kinase C (PKC) in radiation- and H{sub 2}O{sub 2}-evoked c-jun gene expression in human HL-205 cells. This induction of c-jun gene expression could be prevented by pretreatment of the cells with Nacetylcysteine (an antioxidant) or H7 (a PKC and PKA inhibitor) but not by HA1004, a PKA inhibitor, suggesting a role for ROls and PKC in mediating c-jun gene expression. We also investigated potential differences in c-jun gene expression in a panel of normal and tumor cells untreated or treated with ionizing radiation or H{sub 2}O{sub 2}. Treatment with radiation or H{sub 2}O{sub 2} produced a varied response, from some reduction to an increase of more than an order of magnitude in the steady-state level of c-jun mRNA. These data indicate that although induction of c-jun may be a common response to ionizing radiation and H{sub 2}O{sub 2}, this response was reduced or absent in some cell types.

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6 p.

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INIS; OSTI as DE93017436

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  • International seminar on molecular mechanisms in radiation mutagenesis and carcinogenesis, Doorwerth (Netherlands), 19-22 Apr 1993

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  • Other: DE93017436
  • Report No.: ANL/CMB/CP--80231
  • Report No.: CONF-930454--3
  • Grant Number: W-31109-ENG-38
  • Office of Scientific & Technical Information Report Number: 139193
  • Archival Resource Key: ark:/67531/metadc625993

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  • June 1, 1993

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  • June 16, 2015, 7:43 a.m.

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  • Dec. 14, 2015, 12:49 p.m.

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Collart, F.R.; Horio, M. & Huberman, E. c-jun gene expression in human cells exposed to either ionizing radiation or hydrogen peroxide, article, June 1, 1993; Illinois. (digital.library.unt.edu/ark:/67531/metadc625993/: accessed July 23, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.